QPy-TPA is a lipophilic probe that induces non-ferroptotic cell death and regulates lipid dynamics in B16 and HepG2 cells upon light irradiation, with a maximum absorption wavelength of 400 nm and a maximum emission wavelength of 590 nm [1].

QPy-TPA (5 μM) demonstrates phototoxicity in B16 and HepG2 cells, while QPy-TPA (50 μM) maintains a survival rate of over 50% in the absence of light exposure [1]. Under illumination, QPy-TPA increases oxidative lipids, particularly PC and PE [1]. Cell Cytotoxicity Assay [1] Cell Line: B16, HepG2 Concentration: 0-50 μM Incubation Time: 48 h Result: Showed a survival rate of over 50% without light irradiation and reduced cell viabilities in B16 and HepG2 with light irradiation, an effect not reversible by Fer-1.