• Modeling Mechanism：

  Myosin H Chain Fragmen, as an autoantigen, contains pathogenic epitopes in its S2 subunit polymorphic region (amino acids 735-1032). After immunization, it activates autoreactive T cells and antibodies, leading to chronic inflammatory infiltration of the myocardium, myocardial cell necrosis, and ultimately myocarditis. The 838 and 955 amino acid polymorphisms in Myhc of BALB/c mice make them more susceptible to disease, while C57B/6 mice show significant resistance.

• Related Products：

  Myosin H Chain Fragment, Mouse (T78375)

• Modeling Method：

  Experimental Subject：

  Mice, BALB/c (susceptible), C57B/6 (resistant), Female, 4-6 weeks old

  Dosage and Administration Route：

  100 μg mouse cardiac myosin (BALB/c or C57B/6 origin) or rat α-myosin fragment (D0, D4 fragment, containing amino acids 735-1032), Emulsified in complete Freund's adjuvant (CFA), Subcutaneous injection

  Dosing Frequency and Duration Model：

  Immunised once on days 0 and 7

• Validation：

  Histological markers: Chronic inflammatory cell infiltration (predominantly lymphocytes) and cardiomyocyte necrosis were observed in myocardial tissue; the incidence rate in BALB/c mice was significantly higher than in C57B/6 mice. Immunological markers: Anti-myocardial myosin-specific autoantibodies were detected in serum; autoreactive T cells targeting Myhc pathogenic epitopes could be isolated from the spleen.

*Precautions： All mice were sacrificed at predetermined time points after modeling, and their hearts, spleens, and serum were collected for testing.

*References：Liao L,et,al. Cardiac alpha-myosin heavy chains differ in their induction of myocarditis. Identification of pathogenic epitopes. J Clin Invest. 1993 Dec;92(6):2877-82.