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LEI-401

Name: LEI-401
Brand: TargetMol
SKU: T38001
Price: 52 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T38001Cas No. 2393840-15-6

Alias LEI 401

LEI-401 is a selective and potent NAPE-PLD inhibitor that crosses the blood-brain barrier (IC50: 27 nM).LEI-401 reduces the level of n-acylethanolamines (NAEs) in the brain of free-ranging mice and modulates their emotional behaviour.

LEI-401

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Purity: 99.79%

Catalog No. T38001Alias LEI 401Cas No. 2393840-15-6

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
1 mg	$52	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.79%

Appearance:Solid

Color:White

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COA LCMS

Product Introduction

Bioactivity

Description	LEI-401 is a selective and potent NAPE-PLD inhibitor that crosses the blood-brain barrier (IC50: 27 nM).LEI-401 reduces the level of n-acylethanolamines (NAEs) in the brain of free-ranging mice and modulates their emotional behaviour.
Targets&IC50	NAPE-PLD:0.86 μM
In vitro	In HEK293T cells transfected with hNAPE-PLD, LEI-401 (0.04 - 20 μM; 30 minutes) dose-dependently reduces the labeling of NAPE-PLD, with an IC50 of 0.86 μM. LEI-401 decreases the levels of NAE in Neuro-2a cells but does not reduce them in NAPE-PLD KO cells[1].
In vivo	The in vivo pharmacokinetic profile of LEI-401 was evaluated in the plasma of C57BL/6J mice following intraperitoneal (i.p., 30 mg/kg), oral (p.o., 10 mg/kg), or intravenous (i.v., 1 mg/kg) administration. The results demonstrate that LEI-401 is an active NAPE-PLD inhibitor in vivo, indicating its suitability for studying the physiological role of NAPE-PLD[1].
Synonyms	LEI 401

Chemical Properties

Molecular Weight	421.54
Formula	C₂₄H₃₁N₅O₂
Cas No.	2393840-15-6
Smiles	O[C@H]1CCN(C1)c1cc(nc(n1)N1CCC[C@H](C1)c1ccccc1)C(=O)NCC1CC1

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 80 mg/mL (189.78 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+90% Corn Oil: 3.3 mg/mL (7.83 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.3723 mL	11.8613 mL	23.7225 mL	118.6127 mL
5 mM	0.4745 mL	2.3723 mL	4.7445 mL	23.7225 mL
10 mM	0.2372 mL	1.1861 mL	2.3723 mL	11.8613 mL
20 mM	0.1186 mL	0.5931 mL	1.1861 mL	5.9306 mL
50 mM	0.0474 mL	0.2372 mL	0.4745 mL	2.3723 mL
100 mM	0.0237 mL	0.1186 mL	0.2372 mL	1.1861 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc