IDD-8E is an effective anti-pseudomonal agent (MIC = 4.4 µM) with no cytotoxicity. It demonstrates significant killing of Pseudomonas and disruption of its biofilm. IDD-8E targets the ATP-binding pocket of WaaP and also inhibits other ESKAPE pathogens.

IDD-8E, when used at concentrations ranging from 10 to 100 µM for 24 hours in RAW cells, maintains cell viability comparable to untreated controls, indicating no cytotoxicity. At concentrations of 2 and 4.4 µM over 0-8 hours, IDD-8E significantly reduces Pseudomonas aeruginosa numbers, demonstrating an efficacy similar to Rifampicin by inhibiting bacterial growth, and shows potential for biofilm disruption. It exhibits synergistic effects with combinations of antibiotics (Rifampicin, Lincomycin, Carbenicillin), effectively lowering the minimal inhibitory concentrations (MICs) of these antibiotics. IDD-8E also inhibits the growth of other bacterial pathogens, achieving MICs of 6.25 µM for A. baumannii, 50 µM for K. pneumoniae, 12.5 µM for Methicillin-resistant Staphylococcus aureus (MRSA), 12.5 µM for Vancomycin-resistant Enterococcus faecalis (VRE), and 50 µM for E. coli.