HPK1-IN-43 (compound 9f) is an HPK1 kinase inhibitor with an IC50 value of 0.32 nM. It inhibits the phosphorylation of the downstream protein SLP-76 in PBMC cells and enhances the secretion of interleukin 2 (IL-2) and interferon-γ (IFN-γ). HPK1-IN-43 is applicable to cancer research.

HPK1:0.32 nM

HPK1-IN-43 (compound 9f) inhibits the phosphorylation of SLP76 in Jurkat cells and primary peripheral blood mononuclear cells (PBMC) with IC50 values of 147.9 nM and 131.8 nM, respectively, at concentrations ranging from 0-100 nM and durations of 1-18 hours. Additionally, HPK1-IN-43 enhances the production of IL-2 and IFN-γ in PBMC.