Antibody Type: Recombinant Rabbit Monoclonal

Application: WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM

Reactivity: Human,Mouse,Rat (predicted:Zebrafish,Monkey)

IgG

1K734

Human,Mouse,Rat (predicted:Zebrafish,Monkey)

1. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Monoclonal Antibody, Unconjugated (TMAB-00743) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Monoclonal Antibody, Unconjugated (TMAB-00743) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Monoclonal Antibody, Unconjugated (TMAB-00743) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Monoclonal Antibody, Unconjugated (TMAB-00743) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Monoclonal Antibody, Unconjugated (TMAB-00743) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Monoclonal Antibody, Unconjugated (TMAB-00743) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Monoclonal Antibody, Unconjugated (TMAB-00743) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Monoclonal Antibody, Unconjugated (TMAB-00743) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Monoclonal Antibody, Unconjugated (TMAB-00743) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Monoclonal Antibody, Unconjugated (TMAB-00743) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Monoclonal Antibody, Unconjugated (TMAB-00743) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
12. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Monoclonal Antibody, Unconjugated (TMAB-00743) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
13. Paraformaldehyde-fixed, paraffin embedded Rat Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Monoclonal Antibody, Unconjugated (TMAB-00743) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
14. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Monoclonal Antibody, Unconjugated (TMAB-00743) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
15. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Monoclonal Antibody, Unconjugated (TMAB-00743) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
16. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Monoclonal Antibody, Unconjugated (TMAB-00743) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
17. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAPDH Monoclonal Antibody, Unconjugated (TMAB-00743) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
18. 25 μg total protein per Lane of various lysates probed with GAPDH monoclonal antibody, unconjugated (TMAB-00743) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
19. The Hela (H) cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C,the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at room temperature). Primary Antibody (green): Rabbit Anti-GAPDH antibody (TMAB-00743): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488: 1 μg/test. Blank control (black): PBS.
20. The L929 (M) cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C,the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at room temperature). Primary Antibody (green): Rabbit Anti-GAPDH antibody (TMAB-00743): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488: 1 μg/test. Blank control (black): PBS.
21. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at RT for 20 min; Antibody incubation with (GAPDH) monoclonal Antibody, unconjugated (TMAB-00743) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control.
22. 4% Paraformaldehyde-fixed L-929 (M) cell; Triton X-100 at RT for 20 min; Antibody incubation with (GAPDH) monoclonal Antibody, unconjugated (TMAB-00743) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control.

WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM

WB: 1:5000-50000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50-200; IF: 1:100-500; FCM: 1:50-100

Monoclonal

A synthesized peptide: human GAPDH

Human

2597

Energy Metabolism,Huntington's disease,Alzheimer's disease,Neurodegenerative disease,Cancer,Energy Metabolism,Carbohydrate metabolism,GAPDH,Metabolism of carbohydrates