DPPE-PEG550 serves as a PEG lipid functional terminal group used for synthesizing liposomes (LPs) to design conjugated polymer nanoparticles. Liposome nanoparticles (LNPs) are capable of further coupling with other biomolecules through biotin modification and carboxyl terminals. Functionalized nanoparticles can target specific cell proteins for labeling purposes. Using streptavidin as a linker, biotinylated PEG lipid-conjugated polymer nanoparticles can bind with biotinylated antibodies on cell surface receptors, enabling applications in fluorescence-based imaging and sensing.