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Dansylamide

🥰Excellent
Catalog No. T15051Cas No. 1431-39-6

Dansyl amide is a fluorescent dye used in biochemistry and chemistry to label substances with the fluorescent dansyl group.

Dansylamide

Dansylamide

🥰Excellent
Purity: 98.72%
Catalog No. T15051Cas No. 1431-39-6
Dansyl amide is a fluorescent dye used in biochemistry and chemistry to label substances with the fluorescent dansyl group.
Pack SizePriceAvailabilityQuantity
200 mg$33 In Stock
1 mL x 10 mM (in DMSO)$29 In Stock
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Purity:98.72%
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Product Introduction

Bioactivity
Description
Dansyl amide is a fluorescent dye used in biochemistry and chemistry to label substances with the fluorescent dansyl group.
Cell Research
Instructions
I. Reagent preparation
1. Preparation of stock solution: Dissolve it in an appropriate solvent, such as dimethyl sulfoxide (DMSO) or ethanol.
2. Preparation of working solution: The typical working concentration range of Dansylamide is 10 µM to 1 mM, and the specific concentration can be adjusted according to the experimental requirements.
II. Operation steps
1. Labeling process:
1) Sample preparation: Prepare the sample to be labeled, such as protein, peptide or other biomolecule. For protein labeling, it is usually necessary to use a sufficient amount of purified protein.
2) Reaction: Mix the sample with Dansylamide solution and add an appropriate buffer. The pH value of the buffer should usually be controlled at around 8.0 to optimize the labeling process.
3) Incubation: Incubate the sample at room temperature, the reaction time is usually 30 minutes to 2 hours, and the specific time can be adjusted according to the target molecule and the desired degree of labeling.
4) Termination reaction: After incubation, the reaction can be stopped by adding an appropriate terminator or diluting the sample.
2. Purification: After the labeling reaction is completed, dialysis, gel filtration and other methods can be used to remove unreacted Dansylamide to purify the labeled biomolecules.
3. Fluorescence detection:
1) Detection: Detection can be performed using a fluorescence spectrophotometer or fluorescence microscope. The excitation wavelength is set to 335 nm and the emission wavelength is set to 510 nm. The fluorescence intensity is proportional to the molecular weight of the labeled biomolecule.
2) Data analysis: According to the change in fluorescence intensity, a standard curve can be drawn, and the degree of labeling can be evaluated by comparing the sample and the control group.
Notes:
1. Photostability: Dansylamide is sensitive to light, and long-term strong light exposure should be avoided during the experiment to prevent fluorescence attenuation.
2. Concentration control: Too high a labeling concentration may cause autofluorescence quenching, so the dye concentration should be optimized to avoid this.
3. Compatibility: When using with other fluorescent dyes or labeling reagents, ensure that the emission spectra of different dyes do not overlap to avoid signal interference.
Chemical Properties
Molecular Weight250.32
FormulaC12H14N2O2S
Cas No.1431-39-6
SmilesCN(C)c1cccc2c(cccc12)S(N)(=O)=O
Relative Density.1.314g/cm3
Storage & Solubility Information
Storagekeep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice./Shipping at ambient temperature.
Solubility Information
DMSO: 140 mg/mL (559.28 mM), Sonication is recommended.
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM3.9949 mL19.9744 mL39.9489 mL199.7443 mL
5 mM0.7990 mL3.9949 mL7.9898 mL39.9489 mL
10 mM0.3995 mL1.9974 mL3.9949 mL19.9744 mL
20 mM0.1997 mL0.9987 mL1.9974 mL9.9872 mL
50 mM0.0799 mL0.3995 mL0.7990 mL3.9949 mL
100 mM0.0399 mL0.1997 mL0.3995 mL1.9974 mL

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Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH2O. So your working solution concentration is 2 mg/mL。
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2OTargetMol | reagent mix well and clarify
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