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CY5.5-COOH

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Catalog No. T86124Cas No. 1449612-07-0
Alias Cyanine 5.5 carboxylic acid, Cy5.5

CY5.5-COOH (Cyanine 5.5 carboxylic acid) is a fluorescent dye commonly used in biological imaging, cell imaging, protein localization, and immunostaining.

CY5.5-COOH

CY5.5-COOH

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Purity: 99.82%
Catalog No. T86124Alias Cyanine 5.5 carboxylic acid, Cy5.5Cas No. 1449612-07-0
CY5.5-COOH (Cyanine 5.5 carboxylic acid) is a fluorescent dye commonly used in biological imaging, cell imaging, protein localization, and immunostaining.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
1 mg$42In StockIn Stock
5 mg$98In StockIn Stock
10 mg$163In StockIn Stock
25 mg$328In StockIn Stock
50 mg$492In StockIn Stock
100 mg$688-In Stock
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
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Purity:99.82%
Appearance:Solid
Color:Red
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Product Introduction

Bioactivity
Description
CY5.5-COOH (Cyanine 5.5 carboxylic acid) is a fluorescent dye commonly used in biological imaging, cell imaging, protein localization, and immunostaining.
In vitro
Fluorescent labeling of PP microplastics
This experiment uses the CSD method to fluorescently label PP microplastics for biodistribution analysis.
1. Solution preparation: Use DMSO to prepare CY5.5-COOH into a certain mother solution concentration and dilute it to 50 mg/mL for standby use.
Note: The mother solution is stored at -80℃ or -20℃, and the working solution is prepared and used immediately.
2. Operation steps:
1. Add 15g PP microplastics to 150 mL distilled water and 150 mL THF and stir for 10 minutes.
2. Add the diluted working solution CY5.5-COOH (50 mg/mL) to the PP microplastic suspension and stir for 4 days.
3. After the reaction is completed, use qualitative filter paper (2 to 3 µm) to vacuum filter and separate the Cy-PP microplastics to remove unlabeled CY5.5-COOH in the reaction suspension.
4. Wash with distilled water and ethanol.
5. Dry the Cy-PP microplastics in a dark place at 40°C.
6. Analyze the morphology and chemical structure of Cy5.5 labeled PP using SEM and FT-IR spectroscopy, respectively. [1]

The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
SynonymsCyanine 5.5 carboxylic acid, Cy5.5
Chemical Properties
Molecular Weight582.77
FormulaC40H42N2O2
Cas No.1449612-07-0
SmilesO=C([O-])CCCCCN1C=2C=CC=3C=CC=CC3C2C(C1=CC=CC=CC4=[N+](C=5C=CC=6C=CC=CC6C5C4(C)C)C)(C)C
Storage & Solubility Information
Storagekeep away from moisture,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
DMSO: 40 mg/mL (68.64 mM), Sonication is recommended.
H2O: < 1 mg/mL (insoluble)
In Vivo Formulation
10% DMSO+40% PEG300+5% Tween-80+45% Saline: 1.5 mg/mL (2.57 mM), Sonication is recommended.
Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM1.7159 mL8.5797 mL17.1594 mL85.7971 mL
5 mM0.3432 mL1.7159 mL3.4319 mL17.1594 mL
10 mM0.1716 mL0.8580 mL1.7159 mL8.5797 mL
20 mM0.0858 mL0.4290 mL0.8580 mL4.2899 mL
50 mM0.0343 mL0.1716 mL0.3432 mL1.7159 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
TargetMol | Animal experiments For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.
Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSOTargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSOTargetMol | reagent stock solution to 400 μL PEG300TargetMol | reagent and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH2OTargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.
1 Enter information below:
mg/kg
g
μL
2 Enter the in vivo formulation:
% DMSO
%
% Tween 80
% Saline/PBS/ddH2O

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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