CTLA-4 Protein, Human, Recombinant (GST)

Name: CTLA-4 Protein, Human, Recombinant (GST)
Brand: TargetMol
SKU: TMPJ-00108
Rating: 4.5 (1 reviews)

(Synonyms: Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4, CD152, CTLA4) Copy Product Info

Synonyms: Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4, CD152, CTLA4

Catalog No. TMPJ-00108 Copy Product Info

CTLA-4 Protein, Human, Recombinant (GST) is expressed in HEK293 mammalian cells with C-GST tag. The predicted molecular weight is 40-55 KDa and the accession number is P16410.

Pack Size	Price	USA Stock	Global Stock
5 µg	$54	35 days	35 days
10 µg	$85	35 days	35 days
20 µg	$118	35 days	35 days
50 µg	$183	35 days	35 days
100 µg	$287	35 days	35 days
200 µg	$449	35 days	35 days
500 µg	$825	35 days	35 days
1 mg	$1,180	35 days	35 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Immobilized Mouse B7-1-Fc at 5μg/ml (100 μl/well) can bind Human CTLA-4-GST.: Biotinylated by NHS-biotin prior to testing The ED50 of Recombinant Human CTLA-4-GST is 1.17 ug/ml. (Regularly tested)
Description	CTLA-4 Protein, Human, Recombinant (GST) is expressed in HEK293 mammalian cells with C-GST tag. The predicted molecular weight is 40-55 KDa and the accession number is P16410.
Species	Human
Expression System	HEK293 Cells
Tag	C-GST
Accession Number	P16410
Amino Acid	Lys36-Asp161
Construction	Lys36-Asp161
Protein Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Endotoxin	< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4, CD152, CTLA4
Research Background	Cytotoxic Tlymphocyte 4(CTLA-4,CD152), is a type I transmembrane T cell inhibitory molecule that is a member of the Ig superfamily. Human or mouse CTLA4 cDNA encodes 223 amino acids (aa) including a 35 aa signal sequence, a 126 aa extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane (TM) sequence, and a 41 aa cytoplasmic sequence.It is widely expressed with highest levels in lymphoid tissues. CD28 and CTLA-4, together with their ligands, B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T Cells and may play an important role in their functions. Tcell activation through the Tcell receptor and CD28 leads to increased expression of CTLA4.

Chemical Properties

Molecular Weight

40-55 KDa (reducing condition)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.