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CLK8

Name: CLK8
Brand: TargetMol
SKU: T73573
Price: 58 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T73573Cas No. 898920-65-5

CLK8 is a selective CLOCK inhibitor that binds to and interferes with CLOCK activity, disrupts the interaction between CLOCK and BMAL1, and modulates circadian rhythm amplitude.CLK8 can be used to study circadian rhythm-related disorders.

CLK8

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Purity: 98.03%

Catalog No. T73573Cas No. 898920-65-5

Pack Size	Price	Availability	Quantity
1 mg	$58	In Stock

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Purity:98.03%

Resource Download

COA HNMR HPLC MS

Product Introduction

Bioactivity

Description	CLK8 is a selective CLOCK inhibitor that binds to and interferes with CLOCK activity, disrupts the interaction between CLOCK and BMAL1, and modulates circadian rhythm amplitude.CLK8 can be used to study circadian rhythm-related disorders.
In vitro	CLK8 showed no toxicity to U2OS cells with a maximum concentration of 40 μM, maintaining cell viability above 80%. Similarly, CLK8 enhanced the amplitude of Bmal1-d Luc signal in U2OS and NIH 3T3 cells in a dose-dependent manner, and no circadian oscillations were observed in both cell lines[1].
In vivo	CLK8 was administered intraperitoneally to mice at a concentration of 25 mg/kg. The results indicated that CLK8 decreased the levels of CLOCK in the whole-cell lysate of mouse liver, while the levels of BMAL1 and CRY1 remained unchanged [1].

Chemical Properties

Molecular Weight	498.53
Formula	C₂₉H₂₆N₂O₆
Cas No.	898920-65-5
Smiles	O=C1OC2=C(C(OCC(=O)NC=3C=CC=CC3C(=O)N)=CC=4OC(C)(C)CCC42)C(=C1)C=5C=CC=CC5

Storage & Solubility Information

Storage

keep away from direct sunlight,keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 30 mg/mL (60.18 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (4.01 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.0059 mL	10.0295 mL	20.0590 mL	100.2949 mL
5 mM	0.4012 mL	2.0059 mL	4.0118 mL	20.0590 mL
10 mM	0.2006 mL	1.0029 mL	2.0059 mL	10.0295 mL
20 mM	0.1003 mL	0.5015 mL	1.0029 mL	5.0147 mL
50 mM	0.0401 mL	0.2006 mL	0.4012 mL	2.0059 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Cryptochrome protein (CRY)