CD73/5'-Nucleotidase Protein, Human, Recombinant (hFc)

Name: CD73/5'-Nucleotidase Protein, Human, Recombinant (hFc)
Brand: TargetMol
SKU: TMPY-05580
Rating: 4.5 (1 reviews)

(Synonyms: NTE, NT5, NT, eNT, eN, E5NT, CD73, CALJA, 5'-nucleotidase, ecto (CD73)) Copy Product Info

Synonyms: NTE, NT5, NT, eNT, eN, E5NT, CD73, CALJA, 5'-nucleotidase, ecto (CD73)

Catalog No. TMPY-05580 Copy Product Info

CD73 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 84.5 kDa and the accession number is P21589-1.

CD73/5'-Nucleotidase Protein, Human, Recombinant (hFc)

Pack Size	Price	USA Stock	Global Stock
5 µg	$68	7-10 days	7-10 days
10 µg	$108	7-10 days	7-10 days
20 µg	$178	7-10 days	7-10 days
50 µg	$359	7-10 days	7-10 days
100 µg	$696	7-10 days	7-10 days
200 µg	$1,060	7-10 days	7-10 days
500 µg	$2,230	7-10 days	7-10 days
1 mg	$4,530	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Immobilized Human CD73, hFc Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Anti-CD73 Antibody, hFc Tag with the EC50 of 22.6 ng/ml determined by ELISA (QC Test).
Description	CD73 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 84.5 kDa and the accession number is P21589-1.
Species	Human
Expression System	HEK293 Cells
Tag	C-hFc
Accession Number	P21589-1
Construction	Trp27-Lys547
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from 0.22μm filtered solution in 20mM Tris, 120mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	NTE, NT5, NT, eNT, eN, E5NT, CD73, CALJA, 5'-nucleotidase, ecto (CD73)
Research Background	CD73, also known as ecto-5'-nucleotidase, is an enzyme that in humans is encoded by the NT5E gene.CD73 commonly serves to convert AMP to adenosine.The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. A deficiency of CD73 occurs in a variety of immunodeficiency diseases.

Chemical Properties

Molecular Weight

84.5 kDa (Predicted); 85-100 kDa (Due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Recombinant Proteins

Keywords

NT-5NT 5CD-73CD 735'-nucleotidase, ecto

Related Tags: CD73/5'-Nucleotidase Protein, Human, Recombinant (hFc) chemical structure | CD73/5'-Nucleotidase Protein, Human, Recombinant (hFc) molecular weight