c-Kit/CD117 Protein, Mouse, Recombinant (His)

Name: c-Kit/CD117 Protein, Mouse, Recombinant (His)
Brand: TargetMol
SKU: TMPK-00777
Rating: 4.5 (1 reviews)

(Synonyms: SCFR, PBT, KIT, c-kit, ckit, CD117) Copy Product Info

Synonyms: SCFR, PBT, KIT, c-kit, ckit, CD117

Catalog No. TMPK-00777 Copy Product Info

The c-kit proto-oncogen (CD 117) has been shown to be present in several cell types including normal and neoplastic hemopoietic cells. Among normal BM cells, CD117 expression has been found in about half of the CD34 precursors including progenitors committed to the erythroid, granulo-monocytic, and megakaryocytic cell lineages. CD117 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 56.63 kDa and the accession number is P05532-2.

c-Kit/CD117 Protein, Mouse, Recombinant (His)

Pack Size	Price	USA Stock	Global Stock
5 µg	$58	7-10 days	7-10 days
10 µg	$92	7-10 days	7-10 days
20 µg	$148	7-10 days	7-10 days
50 µg	$289	7-10 days	7-10 days
100 µg	$487	7-10 days	7-10 days
200 µg	$883	7-10 days	7-10 days
500 µg	$1,950	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Mouse CD117, His Tag captured on CM5 Chip via anti-his antibody can bind Human SCF, No Tag with an affinity constant of 14.02 μM as determined in SPR assay.
Description	The c-kit proto-oncogen (CD 117) has been shown to be present in several cell types including normal and neoplastic hemopoietic cells. Among normal BM cells, CD117 expression has been found in about half of the CD34 precursors including progenitors committed to the erythroid, granulo-monocytic, and megakaryocytic cell lineages. CD117 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 56.63 kDa and the accession number is P05532-2.
Species	Mouse
Expression System	HEK293 Cells
Tag	C-His
Accession Number	P05532-2
Construction	Ser25-Pro523
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	SCFR, PBT, KIT, c-kit, ckit, CD117
Research Background	The c-kit proto-oncogen (CD 117) has been shown to be present in several cell types including normal and neoplastic hemopoietic cells. Among normal BM cells, CD117 expression has been found in about half of the CD34 precursors including progenitors committed to the erythroid, granulo-monocytic, and megakaryocytic cell lineages.

Chemical Properties

Molecular Weight

56.63 kDa (predicted). Due to glycosylation, the protein migrates to 75-100 kDa based on Tris-Bis PAGE result.

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.