CCP peptide acetate is a synthetic cyclic citrullinated peptide (CCP). CCP peptide acetate can be used as the substrate for detecting anti-CCP antibodies serologically. CCP peptide acetate functions as a target for autoantibodies with a very high specificity for rheumatoid arthritis (RA).

Instructions
1. Material preparation:
1) CCP peptide acetate: Prepare the solution according to the experimental requirements, the concentration is usually 1-10 μg/mL.
2) Serum sample: serum collected from patients.
3) Determined enzyme-labeled substrate system for detecting antibody reactions.
2. Experimental steps:
1) Add CCP peptide acetate solution to the pretreated 96-well plate.
2) Add patient serum sample, usually diluted 1:100 or adjusted according to experimental conditions.
3) Incubate the reaction plate for 1-2 hours.
4) Use appropriate secondary antibody (such as anti-human IgG conjugated to enzyme) for reaction.
5) Add enzyme-labeled substrate, observe color change, and measure absorbance at the corresponding wavelength using a microplate reader.