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Bromothymol Blue

Name: Bromothymol Blue
Brand: TargetMol
SKU: T18930
Price: 35 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T18930Cas No. 76-59-5

Bromothymol Blue, a pH indicator, appears yellow in acidic conditions and blue in alkaline environments.

Bromothymol Blue

😃Good

Purity: 99.51%

Catalog No. T18930Cas No. 76-59-5

Bromothymol Blue, a pH indicator, appears yellow in acidic conditions and blue in alkaline environments.

Pack Size	Price	USA Warehouse	Global Warehouse
2 g	$35	In Stock	In Stock
5 g	$55	-	In Stock
10 g	$79	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Questions

Batch Information

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Purity:99.51%

Color:White to Purple

Resource Download

COA HNMR LCMS

Product Introduction

Bioactivity

Description	Bromothymol Blue, a pH indicator, appears yellow in acidic conditions and blue in alkaline environments.
In vitro	Upon the addition of a Bromothymol Blue solution (0.1%, w/v) to an agar plate, zones surrounding colonies of K. vulgare 07 mutants change their color from blue to yellow because K. vulgare 07 mutants release 2-KLG on agar plates, thereby acidifying surrounding areas around colonies [2].

Chemical Properties

Molecular Weight	624.38
Formula	C₂₇H₂₈Br₂O₅S
Cas No.	76-59-5
Smiles	CC(C)c1cc(c(C)c(Br)c1O)C1(OS(=O)(=O)c2ccccc12)c1cc(C(C)C)c(O)c(Br)c1C
Relative Density.	1.542 g/cm3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: < 0.1 mg/mL (insoluble)

DMSO: 90 mg/mL (144.14 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.6016 mL	8.0079 mL	16.0159 mL	80.0794 mL
5 mM	0.3203 mL	1.6016 mL	3.2032 mL	16.0159 mL
10 mM	0.1602 mL	0.8008 mL	1.6016 mL	8.0079 mL
20 mM	0.0801 mL	0.4004 mL	0.8008 mL	4.0040 mL
50 mM	0.0320 mL	0.1602 mL	0.3203 mL	1.6016 mL
100 mM	0.0160 mL	0.0801 mL	0.1602 mL	0.8008 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc