Antituberculosis agent-10 (Compound 9) demonstrates antibacterial activity against Mycobacterium tuberculosis with a MIC of 0.3 μM. It exerts its antimicrobial effects by targeting the bacterial 50S ribosomal subunit, thereby inhibiting protein synthesis. Additionally, Antituberculosis agent-10 is active when administered orally.

Antituberculosis agent-10 (72 h) shows no inhibitory activity on mitochondrial protein synthesis (MPS) in HepG2 cells, with an IC50 of 150 μM; however, its metabolites exhibit mitochondrial toxicity.

Antituberculosis agent-10 (i.g.; 5-50 mg/kg; Once a day or twice a day) exhibits exceptional permeability and sustained activity in infected C3HeB/FeJ mice. Furthermore, Antituberculosis agent-10 ((i.g.; 4 mg/kg, Twice a day for a week, then once a day thereafter) significantly reduces the volume of tuberculosis lesions in Mtb-infected marmosets, demonstrating potent antibacterial activity.