Anti-VE-Cadherin Polyclonal Antibody 2 is a Rabbit antibody targeting VE-Cadherin. Anti-VE-Cadherin Polyclonal Antibody 2 can be used in FCM,WB.

IgG

Human

1. Sample:
Lane 1: Human MCF-7 cell lysates
Lane 2: Human HUVEC cell lysates
Lane 3: Human A431 cell lysates
Lane 4: Human HeLa cell lysates
Primary: Anti-CD144/VE Cadherin (TMAB-01946) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 86 kDa
Observed band size: 140 kDa
2. Sample:
Lane 1: MCF-7 (Human) Cell Lysate at 30 μg
Lane 2: HUVEC (Human) Cell Lysate at 30 μg
Lane 3: A431 (Human) Cell Lysate at 30 μg
Lane 4: LOVO (Human) Cell Lysate at 30 μg
Lane 5: SiHa (Human) Cell Lysate at 30 μg
Primary: Anti-CD144/VE Cadherin (TMAB-01946) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130 kDa
Observed band size: 130 kDa
3. Blank control: HUVEC.
Primary Antibody (green line): Rabbit Anti-CD144/VE Cadherin antibody (TMAB-01946)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
4. Blank control: HUVC.
Primary Antibody (green line): Rabbit Anti-CD144/VE Cadherin antibody (TMAB-01946)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB: 1:500-2000; FCM: 1ug/Test

Polyclonal

KLH conjugated synthetic peptide: mouse CD144/VE Cadherin

Mouse

CDH5

Cadherin-5

Cadherins,Endothelial Cell Markers,Cell adhesion molecules,Endothelial Cells,Endothelium,Cadherins,Endothelial,Vascular

Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction.