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Anti-VE-Cadherin Polyclonal Antibody 2
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Catalog No. TMAB-01946
Anti-VE-Cadherin Polyclonal Antibody 2 is a Rabbit antibody targeting VE-Cadherin. Anti-VE-Cadherin Polyclonal Antibody 2 can be used in WB,FCM.
Anti-VE-Cadherin Polyclonal Antibody 2
Anti-VE-Cadherin Polyclonal Antibody 2
😃Good
Catalog No. TMAB-01946
Anti-VE-Cadherin Polyclonal Antibody 2 is a Rabbit antibody targeting VE-Cadherin. Anti-VE-Cadherin Polyclonal Antibody 2 can be used in WB,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,FCM Reactivity: Human |
| Ig Type | IgG |
| Reactivity | Human |
| Verified Activity | 1. Sample: Lane 1: Human MCF-7 cell lysates Lane 2: Human HUVEC cell lysates Lane 3: Human A431 cell lysates Lane 4: Human HeLa cell lysates Primary: Anti-CD144/VE Cadherin (TMAB-01946) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 86 kDa Observed band size: 140 kDa 2. Sample: Lane 1: MCF-7 (Human) Cell Lysate at 30 μg Lane 2: HUVEC (Human) Cell Lysate at 30 μg Lane 3: A431 (Human) Cell Lysate at 30 μg Lane 4: LOVO (Human) Cell Lysate at 30 μg Lane 5: SiHa (Human) Cell Lysate at 30 μg Primary: Anti-CD144/VE Cadherin (TMAB-01946) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130 kDa Observed band size: 130 kDa 3. Blank control: HUVEC. Primary Antibody (green line): Rabbit Anti-CD144/VE Cadherin antibody (TMAB-01946) Dilution: 1 μg/Test; Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488 Dilution: 0.5 μg/Test. Isotype control (orange line): Normal Rabbit IgG Protocol The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. 4. Blank control: HUVC. Primary Antibody (green line): Rabbit Anti-CD144/VE Cadherin antibody (TMAB-01946) Dilution: 1 μg/Test; Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488 Dilution: 0.5 μg/Test. Isotype control (orange line): Normal Rabbit IgG Protocol The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. |
| Application | WB,FCM |
| Recommended Dose | WB: 1:500-2000; FCM: 1ug/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell junction. Cell membrane. Found at cell-cell boundaries and probably at cell-matrix boundaries. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq, Jul 2008]. |
| Immunogen | KLH conjugated synthetic peptide: mouse CD144/VE Cadherin |
| Antigen Species | Mouse |
| Gene ID | 12562 |
| Uniprot ID | |
| Biology Area | Cadherins,Endothelial Cell Markers,Cell adhesion molecules,Endothelial Cells,Endothelium,Cadherins,Endothelial,Vascular |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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