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Anti-Uromodulin/Umod Polyclonal Antibody
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Catalog No. TMAB-01939
Alias Uromodulin, Umod, Tamm-Horsfall urinary glycoprotein (THP)
Anti-Uromodulin/Umod Polyclonal Antibody is a Rabbit antibody targeting Uromodulin/Umod. Anti-Uromodulin/Umod Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Uromodulin/Umod Polyclonal Antibody
Anti-Uromodulin/Umod Polyclonal Antibody
😃Good
Catalog No. TMAB-01939Alias Uromodulin, Umod, Tamm-Horsfall urinary glycoprotein (THP)
Anti-Uromodulin/Umod Polyclonal Antibody is a Rabbit antibody targeting Uromodulin/Umod. Anti-Uromodulin/Umod Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Uromodulin/Umod Polyclonal Antibody is a Rabbit antibody targeting Uromodulin/Umod. Anti-Uromodulin/Umod Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | Uromodulin, Umod, Tamm-Horsfall urinary glycoprotein (THP) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Sample: Lane 1: Recombinant human UMOD protein, N-His Primary: Anti-UMOD (TMAB-01939) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 69 kDa Observed band size: 37 kDa 2. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with UMOD Polyclonal Antibody, Unconjugated (TMAB-01939) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with UMOD Polyclonal Antibody, Unconjugated (TMAB-01939) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:400-800; IHC-Fr: 1:400-800; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Apical cell membrane; Lipid-anchor, GPI-anchor (By similarity). Basolateral cell membrane; Lipid-anchor, GPI-anchor (By similarity). Cell projection, cilium membrane (By similarity). Note=Only a small fraction is sorts to the basolateral pole of tubular epithelial cells compared to apical localization (By similarity).Uromodulin, secreted form: Secreted (By similarity). |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The protein encoded by this gene is the most abundant protein in mammalian urine under physiological conditions. Its excretion in urine follows proteolytic cleavage of the ectodomain of its glycosyl phosphatidylinosital-anchored counterpart that is situated on the luminal cell surface of the loop of Henle. This protein may act as a constitutive inhibitor of calcium crystallization in renal fluids. Excretion of this protein in urine may provide defense against urinary tract infections caused by uropathogenic bacteria. Defects in this gene are associated with the renal disorders medullary cystic kidney disease-2 (MCKD2), glomerulocystic kidney disease with hyperuricemia and isosthenuria (GCKDHI), and familial juvenile hyperuricemic nephropathy (FJHN). Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2013]. |
| Immunogen | KLH conjugated synthetic peptide: mouse UMOD |
| Antigen Species | Mouse |
| Biology Area | Other Antibodies |
| Molecular Weight | Theoretical: 61/65 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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