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Anti-Uromodulin/Umod Polyclonal Antibody

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Catalog No. TMAB-01939

Anti-Uromodulin/Umod Polyclonal Antibody is a Rabbit antibody targeting Uromodulin/Umod. Anti-Uromodulin/Umod Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-Uromodulin/Umod Polyclonal Antibody

Anti-Uromodulin/Umod Polyclonal Antibody

😃Good
Catalog No. TMAB-01939
Anti-Uromodulin/Umod Polyclonal Antibody is a Rabbit antibody targeting Uromodulin/Umod. Anti-Uromodulin/Umod Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$221 7-10 days
100 μL$372 7-10 days
200 μL$528 7-10 days
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Product Introduction

Bioactivity
Description
Anti-Uromodulin/Umod Polyclonal Antibody is a Rabbit antibody targeting Uromodulin/Umod. Anti-Uromodulin/Umod Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse,Rat
Verified Activity
1. Sample:
Lane 1: Recombinant human UMOD protein, N-His
Primary: Anti-UMOD (TMAB-01939) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 69 kDa
Observed band size: 37 kDa
2. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with UMOD Polyclonal Antibody, Unconjugated (TMAB-01939) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with UMOD Polyclonal Antibody, Unconjugated (TMAB-01939) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:400-800; IHC-Fr: 1:400-800; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationApical cell membrane; Lipid-anchor, GPI-anchor (By similarity). Basolateral cell membrane; Lipid-anchor, GPI-anchor (By similarity). Cell projection, cilium membrane (By similarity). Note=Only a small fraction is sorts to the basolateral pole of tubular epithelial cells compared to apical localization (By similarity).Uromodulin, secreted form: Secreted (By similarity).
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe protein encoded by this gene is the most abundant protein in mammalian urine under physiological conditions. Its excretion in urine follows proteolytic cleavage of the ectodomain of its glycosyl phosphatidylinosital-anchored counterpart that is situated on the luminal cell surface of the loop of Henle. This protein may act as a constitutive inhibitor of calcium crystallization in renal fluids. Excretion of this protein in urine may provide defense against urinary tract infections caused by uropathogenic bacteria. Defects in this gene are associated with the renal disorders medullary cystic kidney disease-2 (MCKD2), glomerulocystic kidney disease with hyperuricemia and isosthenuria (GCKDHI), and familial juvenile hyperuricemic nephropathy (FJHN). Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2013].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: mouse UMOD
Antigen Species
Mouse
Biology Area
Other Antibodies
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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