Anti-Tyrosine Hydroxylase Polyclonal Antibody is a Rabbit antibody targeting Tyrosine Hydroxylase. Anti-Tyrosine Hydroxylase Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Mouse,Rat

1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Tyrosine Hydroxylase Polyclonal Antibody, Unconjugated (TMAB-01924) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Tyrosine Hydroxylase) Polyclonal Antibody, Unconjugated (TMAB-01924) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 90 minutes, and DAPI for nucleus staining.
3. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Tyrosine Hydroxylase) Polyclonal Antibody, Unconjugated (TMAB-01924) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 90 minutes, and DAPI for nucleus staining.
4. Sample: PC-3 (Mouse) Lysate at 30 μg
Primary: Anti-Tyrosine Hydroxylase (TMAB-01924) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit Igg (secondary antibody) at 1:5000 dilution;
Predicted band size:60 kDa
Observed band size:60 kDa
5. Sample: adipocyte (mouse) Lysate at 5-10 μg
model A, model B, model C are from different mice;
Primary: Anti-Tyrosine Hydroxylase (TMAB-01924) at 1/2000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
6. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Tyrosine Hydroxylase) Polyclonal Antibody, Unconjugated (TMAB-01924) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Tyrosine Hydroxylase) Polyclonal Antibody, Unconjugated (TMAB-01924) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human TH

Human

Noradrenaline,Dopamine,Soma marker,Hypoxia,Hormone biosynthesis,Response to hypoxia,Hormone biosynthesis