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Anti-TIE2 Polyclonal Antibody

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Catalog No. TMAB-01839
Alias Tie-2, Tie2, TEK tyrosine kinase, endothelial, STK1, Hyk, Cd202b, AA517024

Anti-TIE2 Polyclonal Antibody is a Rabbit antibody targeting TIE2. Anti-TIE2 Polyclonal Antibody can be used in ICC/IF,FCM.

Anti-TIE2 Polyclonal Antibody

Anti-TIE2 Polyclonal Antibody

😃Good
Catalog No. TMAB-01839Alias Tie-2, Tie2, TEK tyrosine kinase, endothelial, STK1, Hyk, Cd202b, AA517024
Anti-TIE2 Polyclonal Antibody is a Rabbit antibody targeting TIE2. Anti-TIE2 Polyclonal Antibody can be used in ICC/IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3747-10 days
200 μL$5277-10 days
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Product Introduction

Bioactivity
Description
Anti-TIE2 Polyclonal Antibody is a Rabbit antibody targeting TIE2. Anti-TIE2 Polyclonal Antibody can be used in ICC/IF,FCM.
AliasTie-2, Tie2, TEK tyrosine kinase, endothelial, STK1, Hyk, Cd202b, AA517024
Ig Type
IgG
Reactivity
Human,Rat (predicted:Mouse,Pig,Cow,Horse)
Verified Activity
1. Tissue/cell: A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (TIE2) polyclonal Antibody, Unconjugated (TMAB-01839) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
2. Blank control (Black line): HUVEC (Black). Primary Antibody (green line): Rabbit Anti-TIE2 antibody (TMAB-01839) Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
Application
ICC/IF,FCM
Recommended Dose
ICC/IF: 1:100; FCM: 3ug/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane; Single-pass type I membrane protein. Cell junction. Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Secreted. Note=Recruited to cell-cell contacts in quiescent endothelial cells. Colocalizes with the actin cytoskeleton and at actin stress fibers during cell spreading. Recruited to the lower surface of migrating cells, especially the rear end of the cell. Proteolytic processing gives rise to a soluble extracellular domain that is secreted.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells in mice, rats, and humans. This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. The ligand for the receptor is angiopoietin-1. Defects in TEK are associated with inherited venous malformations; the TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis.TEK is closely related to the TIE receptor tyrosine kinase.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Tie2
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Endothelial Markers,Receptor Tyrosine Kinases,Angiogenesis and vasculogenesis,Endothelium,Endothelial Cells,Endothelial Cell Markers,Growth factor receptors
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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