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Anti-RAC1/2/3 Antibody (1M237)
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Catalog No. TMAC-03498
Alias TC-25, ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1), Rac-1, p21-Rac1, MIG5
Anti-RAC1/2/3 Antibody (1M237) is a Rabbit antibody targeting RAC1/2/3. Anti-RAC1/2/3 Antibody (1M237) can be used in WB,ICC/IF,IHC.
Anti-RAC1/2/3 Antibody (1M237)
Anti-RAC1/2/3 Antibody (1M237)
😃Good
Catalog No. TMAC-03498Alias TC-25, ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1), Rac-1, p21-Rac1, MIG5
Anti-RAC1/2/3 Antibody (1M237) is a Rabbit antibody targeting RAC1/2/3. Anti-RAC1/2/3 Antibody (1M237) can be used in WB,ICC/IF,IHC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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| Description | Anti-RAC1/2/3 Antibody (1M237) is a Rabbit antibody targeting RAC1/2/3. Anti-RAC1/2/3 Antibody (1M237) can be used in WB,ICC/IF,IHC. |
| Alias | TC-25, ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1), Rac-1, p21-Rac1, MIG5 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of RAC1/2/3 on MCF-7 cells lysates using anti-RAC1/2/3 antibody at 1/500 dilution. 2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-RAC1/2/3 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-RAC1/2/3 antibody. Counter stained with hematoxylin. 4. ICC staining RAC1/2/3 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining RAC1/2/3 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining RAC1/2/3 in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC: 1:50-200; ICC/IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages (By similarity). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Molecular Weight | Theoretical: 21/24 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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