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Anti-NSD3 Antibody (1A959) is a Rabbit antibody targeting NSD3. Anti-NSD3 Antibody (1A959) can be used in WB,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $297 | 7-10 days | |
100 μL | $497 | 7-10 days |
Description | Anti-NSD3 Antibody (1A959) is a Rabbit antibody targeting NSD3. Anti-NSD3 Antibody (1A959) can be used in WB,FCM. |
Synonyms | Wolf-Hirschhorn syndrome candidate 1-like protein 1, Wolf Hirschhorn syndrome candidate 1 like 1, WHSC1-like protein 1, WHSC1-like 1 isoform 9 with methyltransferase activity to lysine, WHSC1L1 protein, WHSC1L1, WHSC1 like protein 1, Whistle, Protein whistle, pp14328, Nuclear SET domain-containing protein 3, Nuclear SET domain containing protein 3, Nuclear set domain containing 3, NSD3_HUMAN, NSD 3, MGC142029, MGC126766, Histone-lysine N-methyltransferase NSD3, Histone lysine N methyltransferase NSD3, FLJ20353, DKFZp667H044 |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Western blot analysis of NSD3 on different lysates using anti-NSD3 antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7, Lane 2: 293T. 2. Flow cytometric analysis of Hela cells with NSD3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
Application | |
Recommended Dose | WB: 1:1000-2000; FCM: 1:50-100 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | Recombinant Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | The deduced 1,437 amino acid NSD3 protein contains two PWWP domains involved in protein-protein interactions, five PHD-type zinc finger motifs found in chromatin-associated proteins, a SAC (SET-associated cys-rich) domain, a SET domain and a C-terminal C5HCH domain. Two NSD3 variants have been identified. The short variant comprised of 645 amino acids, arises from alternative polyadenylation and exon splicing and contains a single PWWP domain. A longer NSD3 variant, which is only expressed in HeLa cells, is comprised of 1,388 amino acid residues. The human WHSC1L1 gene, which encodes the NSD3 protein, shares 68% and 55% identity with mouse Nsd1 and human WHSC1, respectively. Highest expression of NSD3 is observed in brain, heart and skeletal muscle tissues; lower levels of NSD3 expression are observed in the liver and lungs. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein |
Uniprot ID |
Molecular Weight | Theoretical: 162 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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