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Anti-N Cadherin Antibody-PE (3E575)
🥰Excellent
Catalog No. TMAY-01507P
Anti-N Cadherin Antibody-PE (3E575) is a PE-conjugated Rabbit antibody targeting N Cadherin. Anti-N Cadherin Antibody-PE (3E575) can be used in FCM.
Anti-N Cadherin Antibody-PE (3E575)
Anti-N Cadherin Antibody-PE (3E575)
🥰Excellent
Catalog No. TMAY-01507P
Anti-N Cadherin Antibody-PE (3E575) is a PE-conjugated Rabbit antibody targeting N Cadherin. Anti-N Cadherin Antibody-PE (3E575) can be used in FCM.
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-N Cadherin Antibody-PE (3E575) is a PE-conjugated Rabbit antibody targeting N Cadherin. Anti-N Cadherin Antibody-PE (3E575) can be used in FCM. |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 3E575 |
| Reactivity | Human |
| Verified Activity | Flow cytometry analysis of N-Cadherin on HeLa cells. HeLa cells were harvested with (Left) or without (Right) trypsinization (please note, the epitope is sensitive to trypsin) and stained with either Rabbit IgG isotype control FITC (dashed line) or Rabbit Anti-Human CD325 antibody PE (solid line) at matching concentrations. |
| Application | FCM |
| Recommended Dose | 10 μl/Test, 0.1 mg/ml |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human N-Cadherin / CD325 / CDH2 (rh N-Cadherin / CD325 / CDH2; TMPY-01143; NP_001783.2; Met1-Ala724) and conjugated with PE under optimum conditions, the unreacted PE was removed. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | PBS solution containing 0.5% BSA and 0.09% sodium azide |
| Research Background | Cadherins are calcium-dependent cell adhesion proteins, and they preferentially interact with themselves in a homophilic manner in connecting cells. Cadherin 2 (CDH2), also known as N-Cadherin (neuronal) (NCAD), is a single-pass transmembrane protein and a cadherin containing 5 cadherin domains. N-Cadherin displays a ubiquitous expression pattern but with different expression levels between endocrine cell types. CDH2 (NCAD) has been shown to play an essential role in normal neuronal development, which is implicated in an array of processes including neuronal differentiation and migration, and axon growth and fasciculation. In addition, N-Cadherin expression was upregulated in human HSC during activation in culture, and function or expression blocking of N-Cadherin promoted apoptosis. During apoptosis, N-Cadherin was cleaved into 20-100 kDa fragments. It may provide a novel target for therapies that are directed toward intimal proliferative disorders, including restenosis and vascular bypass graft failure. N-Cadherin is associated with tumor aggressiveness and metastatic potential and may contribute to tumor progression. |
| Conjucates | PE |
| Others Formats | Unconjugated/APC/FITC |
| Antibody Types Available | 4 |
| Immunogen | Recombinant Human N-Cadherin / CD325 / CDH2 protein (TMPY-01143) |
| Antigen Species | Human |
| Biology Area | Early Mesodermal Lineage Markers |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 12 months, do not freeze. Keep away from direct sunlight. Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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