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Anti-DPP4/CD26 Polyclonal Antibody
😃Good
Catalog No. TMAB-00567
Alias dipeptidylpeptidase 4
Anti-DPP4/CD26 Polyclonal Antibody is a Rabbit antibody targeting DPP4/CD26. Anti-DPP4/CD26 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
Anti-DPP4/CD26 Polyclonal Antibody
Anti-DPP4/CD26 Polyclonal Antibody
😃Good
Catalog No. TMAB-00567Alias dipeptidylpeptidase 4
Anti-DPP4/CD26 Polyclonal Antibody is a Rabbit antibody targeting DPP4/CD26. Anti-DPP4/CD26 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-DPP4/CD26 Polyclonal Antibody is a Rabbit antibody targeting DPP4/CD26. Anti-DPP4/CD26 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM. |
| Alias | dipeptidylpeptidase 4 |
| Ig Type | IgG |
| Reactivity | Human,Rat (predicted:Mouse,Pig,Cow,Horse,Rabbit) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Human melanoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD26) Polyclonal Antibody, Unconjugated (TMAB-00567) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Blank control (blue): Raji (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-CD26 antibody (TMAB-00567), Dilution: 3 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. 3. Paraformaldehyde-fixed, paraffin embedded (rat intestine tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD26) Polyclonal Antibody, Unconjugated (TMAB-00567) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 3μg/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane. Apical cell membrane. Cell projection > invadopodium membrane. Cell projection > lamellipodium membrane. Cell junction. Membrane raft. Translocated to the apical membrane through the concerted action of N- and O-Glycans and its association with lipid microdomains containing cholesterol and sphingolipids. Redistributed to membrane rafts in T-cell in a interleukin-12-dependent activation. Its interaction with CAV1 is necessary for its translocation to membrane rafts. Colocalized with PTPRC in membrane rafts. Colocalized with FAP in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with FAP on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. Colocalized with ADA at the cell junction in lymphocyte-epithelial cell adhesion. Colocalized with IGF2R in internalized cytoplasmic vesicles adjacent to the cell surface and Secreted. Detected in the serum and the seminal fluid. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The DPP4 gene encodes dipeptidyl peptidase 4, which is identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. It is an intrinsic type II transmembrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. Dipeptidyl peptidase 4 is highly involved in glucose and insulin metabolism, as well as in immune regulation. This protein was shown to be a functional receptor for Middle East respiratory syndrome coronavirus (MERS-CoV), and protein modeling suggests that it may play a similar role with SARS-CoV-2, the virus responsible for COVID-19. [provided by RefSeq, Apr 2020] |
| Immunogen | KLH conjugated synthetic peptide: human CD26 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Response to hypoxia,DPPs,CD,CD,Adhesion,Non-lineage,HIV-related,Hypoxia,SARS Coronavirus,B Lymphocytic Lineage,T Lymphocytic Lineage |
| Molecular Weight | Theoretical: 84 kDa. Actual: 110 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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