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Anti-Cyclin A1 Antibody (2E766)
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Catalog No. TMAY-01463
Alias cyclin A1, CT146
Anti-Cyclin A1 Antibody (2E766) is a Mouse antibody targeting Cyclin A1. Anti-Cyclin A1 Antibody (2E766) can be used in FCM,ICC/IF.
Anti-Cyclin A1 Antibody (2E766)
Anti-Cyclin A1 Antibody (2E766)
😃Good
Catalog No. TMAY-01463Alias cyclin A1, CT146
Anti-Cyclin A1 Antibody (2E766) is a Mouse antibody targeting Cyclin A1. Anti-Cyclin A1 Antibody (2E766) can be used in FCM,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $199 | 7-10 days |
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Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-Cyclin A1 Antibody (2E766) is a Mouse antibody targeting Cyclin A1. Anti-Cyclin A1 Antibody (2E766) can be used in FCM,ICC/IF. |
| Alias | cyclin A1, CT146 |
| Ig Type | Mouse IgG2b |
| Clone | 2E766 |
| Reactivity | Human |
| Verified Activity | 1. Immunofluorescence staining of Human CCNA1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human CCNA1 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and Nucleus. 2. Flow cytometric analysis of Human CCNA1 expression on HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human CCNA1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Application | FCM,ICC/IF |
| Recommended Dose | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Cyclin A1 / CCNA1 (rh Cyclin A1 / CCNA1; TMPY-01401; NP_003905.1; Met1-Gln465). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Cyclin A1 is a member of the highly conserved cyclin family that is characterized by a dramatic periodicity in protein abundance, and belongs to the A-type cyclin subfamily. The mammalian A-type cyclin family consists of two members: cyclin A1 and cyclin A2. Different cyclins exhibit distinct expression. Cyclin A1 is expressed in mice exclusively in the germ cell lineage and high rate of cyclinA1 is found in human testis and certain myeloid leukaemia cells. Cyclin A1 is primarily function in the control of meiosis. It serves as regulator subunits binding to cyclin-dependent kinase 1 (Cdk1) and cyclin-dependent kinase 2 (Cdk2), which give two different kinase activities, one appearing in S phase, the other in G2. Through this, cyclin A1 operate the entry and progression in cell cycle. High frequency of cyclin A1 overexpression has been observed in acute myelocytic leukemias, especially those that are at the promyelocyte and myeloblast stages of development. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human Cyclin A1 / CCNA1 protein (TMPY-01401) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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