Anti-CTH Polyclonal Antibody is a Rabbit antibody targeting CTH. Anti-CTH Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse (predicted:Rat,Dog,Cow,Rabbit)

1. Sample: K562 Cell (Human) Lysate at 40 μg
Primary: Anti-CTH (TMAB-00490) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
2. Sample: Esophagus (Mouse) Lysate at 40 μg
Primary: Anti-CTH (TMAB-00490) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
3. Sample: Liver (Mouse) Lysate at 40 μg
Primary: Anti-CTH (TMAB-00490) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 48 kDa
4. Blank control: U937. Primary Antibody (green line): Rabbit Anti-CTH antibody (TMAB-00490)
Dilution: 2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB,FCM

WB: 1:500-2000; FCM: 2ug/Test

Polyclonal

KLH conjugated synthetic peptide: human CTH/CSE/Cystathionase

Human

Amino acid metabolism,Amino Acids