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Anti-CLEC4E Polyclonal Antibody

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Catalog No. TMAB-00446

Anti-CLEC4E Polyclonal Antibody is a Rabbit antibody targeting CLEC4E. Anti-CLEC4E Polyclonal Antibody can be used in FCM.

Anti-CLEC4E Polyclonal Antibody

Anti-CLEC4E Polyclonal Antibody

😃Good
Catalog No. TMAB-00446
Anti-CLEC4E Polyclonal Antibody is a Rabbit antibody targeting CLEC4E. Anti-CLEC4E Polyclonal Antibody can be used in FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3737-10 days
200 μL$5297-10 days
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Product Introduction

Bioactivity
Description
Anti-CLEC4E Polyclonal Antibody is a Rabbit antibody targeting CLEC4E. Anti-CLEC4E Polyclonal Antibody can be used in FCM.
Ig Type
IgG
Reactivity
Human (predicted:Horse,Cow,Rat,Mouse)
Verified Activity
1. Blank control: Hela (blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.
Isotype Control Antibody: Rabbit Igg (orange);
Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue),
Dilution: 1:200 in 1 X PBS containing 0.5% BSA;
Primary Antibody Dilution: 5 μg in 100 μL 1X PBS containing 0.5% BSA (green).
2. Black line: Positive blank control (Hela); Negative blank control (A431)
Green line: Primary Antibody (Rabbit Anti-CLEC 4E antibody (TMAB-00446))
Orange line: Isotype Control Antibody (Rabbit IgG).
Blue line: Secondary Antibody (Goat anti-rabbit IgG-PE)/(Goat anti-rabbit IgG-AF488)
Hela (Positive) and A431 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CLEC 4E Antibody (TMAB-00446) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody (blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
3. Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-CLEC 4E antibody (TMAB-00446)
Dilution: 2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
Application
FCM
Recommended Dose
FCM: 2μg/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMembrane.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundC-type lectin that functions as cell-surface receptor for a wide variety of ligands such as damaged cells, fungi and mycobacteria. Plays a role in the recognition of pathogenic fungi, such as Candida albicans. The detection of mycobacteria is via trehalose 6,6'-dimycolate (TDM), a cell wall glycolipid. Specifically recognizes alpha-mannose residues on pathogenic fungi of the genus Malassezia. Recognizes also SAP130, a nuclear protein, that is released by dead or dying cells. Transduces signals through an ITAM-containing adapter protein, Fc receptor gamma chain /FCER1G. Induces secretion of inflammatory cytokines through a pathway that depends on SYK, CARD9 and NF-kappa-B.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human CLECSF9
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Macrophage / Inflamm.
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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