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Anti-CDK1 Polyclonal Antibody is a Rabbit antibody targeting CDK1. Anti-CDK1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $220 | 7-10 days | |
100 μL | $373 | 7-10 days | |
200 μL | $529 | 7-10 days |
Description | Anti-CDK1 Polyclonal Antibody is a Rabbit antibody targeting CDK1. Anti-CDK1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
Ig Type | IgG |
Reactivity | Human,Mouse (predicted:Rat,Dog,Horse) |
Verified Activity | 1. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (CDK1) polyclonal Antibody, Unconjugated (TMAB-00402) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. 2. Sample: U251 (Human) Cell Lysate at 30 μg A673 (Human) Cell Lysate at 30 μg MCF-7 (Human) Cell Lysate at 30 μg MDA-MB-231 (Human) Cell Lysate at 30 μg Primary: Anti-CDK1 (TMAB-00402) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kDa Observed band size: 34 kDa 3. Tissue/cell: human breast carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-CDK1 Polyclonal Antibody, Unconjugated (TMAB-00402) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 4. Tissue/cell: human laryngocarcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-CDK1 Polyclonal Antibody, Unconjugated (TMAB-00402) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 5. Sample: Jurkat (Human) Cell Lysate at 30 μg Primary: Anti-CDK1 (TMAB-00402) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kDa Observed band size: 33 kDa 6. Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CDK1) Polyclonal Antibody, Unconjugated (TMAB-00402) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CDK1) Polyclonal Antibody, Unconjugated (TMAB-00402) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
Application | WB,IHC-P,IHC-Fr,ICC/IF,IF |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Nucleus. Cytoplasm. Mitochondrion. Cytoplasm, cytoskeleton, centrosome. Note=Cytoplasmic during the interphase. Reversibly translocated from cytoplasm to nucleus when phosphorylated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] |
Immunogen | KLH conjugated synthetic peptide: human CDK1 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Cdks,Cdks,Neural Signal Transduction,Neurodegenerative disease |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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