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Anti-CD3 epsilon/CD3e Polyclonal Antibody 2
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Catalog No. TMAB-00352
Alias T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e
Anti-CD3 epsilon/CD3e Polyclonal Antibody 2 is a Rabbit antibody targeting CD3 epsilon/CD3e. Anti-CD3 epsilon/CD3e Polyclonal Antibody 2 can be used in IF, IHC-Fr, IHC-P, WB.
Anti-CD3 epsilon/CD3e Polyclonal Antibody 2
Anti-CD3 epsilon/CD3e Polyclonal Antibody 2
😃Good
Catalog No. TMAB-00352Alias T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e
Anti-CD3 epsilon/CD3e Polyclonal Antibody 2 is a Rabbit antibody targeting CD3 epsilon/CD3e. Anti-CD3 epsilon/CD3e Polyclonal Antibody 2 can be used in IF, IHC-Fr, IHC-P, WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-CD3 epsilon/CD3e Polyclonal Antibody 2 is a Rabbit antibody targeting CD3 epsilon/CD3e. Anti-CD3 epsilon/CD3e Polyclonal Antibody 2 can be used in IF, IHC-Fr, IHC-P, WB. |
| Synonyms | T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e |
| Ig Type | IgG |
| Reactivity | Mouse (predicted:Human,Rat) |
| Verified Activity | 1. Tissue/cell: mouse lymphoma tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-CD3 Polyclonal Antibody, Unconjugated (TMAB-00352) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated used at 1:200 dilution for 40 minutes at 37°C. 2. Sample: Lane 1: Recombinant mouse CD3E protein, C-mFc (HEK293) Primary: Anti-CD3E (TMAB-00352) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20 kDa Observed band size: 36 kDa   |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane; Single-pass type I membrane protein. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | CD3e molecule, epsilon is also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2. |
| Immunogen | KLH conjugated synthetic peptide: mouse CD3E |
| Antigen Species | Mouse |
| Gene Name | CD3E |
| Gene ID | |
| Protein Name | T-cell surface glycoprotein CD3 epsilon chain |
| Uniprot ID | |
| Biology Area | Regulatory T Cells,CD,cd3,Human Lineage Negative |
| Function | The CD3 complex mediates signal transduction. |
| Molecular Weight | Theoretical: 20 kDa. Actual: 23-26 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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