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Anti-CD27/TNFRSF7 Antibody (4I709)
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Catalog No. TMAY-03108
Alias Tp55, Tnfrsf7, S152, CD27 molecule
Anti-CD27/TNFRSF7 Antibody (4I709) is a Rabbit antibody targeting CD27/TNFRSF7. Anti-CD27/TNFRSF7 Antibody (4I709) can be used in FCM,ICC/IF.
Anti-CD27/TNFRSF7 Antibody (4I709)
Anti-CD27/TNFRSF7 Antibody (4I709)
😃Good
Catalog No. TMAY-03108Alias Tp55, Tnfrsf7, S152, CD27 molecule
Anti-CD27/TNFRSF7 Antibody (4I709) is a Rabbit antibody targeting CD27/TNFRSF7. Anti-CD27/TNFRSF7 Antibody (4I709) can be used in FCM,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $269 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: FCM, ICC/IF Reactivity: Mouse |
| Alias | Tp55, Tnfrsf7, S152, CD27 molecule |
| Ig Type | Rabbit IgG |
| Clone | 4I709 |
| Reactivity | Mouse |
| Verified Activity | 1. Immunofluorescence staining of Mouse CD27 in Mouse Spleen cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse CD27 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane. 2. Expression of CD27 on mouse splenocytes. BABL/c splenocytes were stained with purified mAb R012, then a FITC-conjugated second step antibody. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system. |
| Application | FCM,ICC/IF |
| Recommended Dose | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD27 (rM CD27; TMPY-01432; NP_001028298.1; Met1-Arg182). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a type I transmembrane glycoprotein of about 55 kDa existing as disulfide-linked homodimer, CD27 has been shown to play roles in lymphoid proliferation, differentiation, and apoptosis.It has an important role in the generation of T cell immunity and is an robust marker for normal memory B cells. It is a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis.Cancer ImmunotherapyCo-stimulatory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: FCM AntibodiesImmune Checkpoint Detection: ICC AntibodiesImmune Checkpoint Detection: IHC AntibodiesImmune Checkpoint ProteinsImmune Checkpoint TargetsImmunotherapyTargeted Therapy |
| Conjucates | Unconjugated |
| Others Formats | APC/PerCP/FITC/PE |
| Antibody Types Available | 5 |
| Immunogen | Recombinant Protein: Mouse CD27 protein (TMPY-01432) |
| Antigen Species | Mouse |
| Biology Area | Cancer Drug Targets |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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