Anti-C5b-9 Polyclonal Antibody is a Rabbit antibody targeting C5b-9. Anti-C5b-9 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Human,Rat (predicted:Mouse)

1. Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (C5b-9) Polyclonal Antibody, Unconjugated (TMAB-00264) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
2. Sample:
Lane 1: A549 (Human) Cell Lysate at 30 μg
Lane 2: Hela (Human) Cell Lysate at 30 μg
Primary: Anti-C5b (TMAB-00264) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 72 kDa
Observed band size: 72 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

Purified Human SC5b-9 Complex

Human

C5

Complement C5

Oxidative Stress,Alternative Pathway,Classical Pathway,MAC,Macrophage / Inflamm.,Oxidative stress,GPCR

Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.
Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C5a also stimulates the locomotion of polymorphonuclear leukocytes (chemokinesis) and direct their migration toward sites of inflammation (chemotaxis).