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Anti-C5b-9 Polyclonal Antibody
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Catalog No. TMAB-00264
Anti-C5b-9 Polyclonal Antibody is a Rabbit antibody targeting C5b-9. Anti-C5b-9 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-C5b-9 Polyclonal Antibody
Anti-C5b-9 Polyclonal Antibody
😃Good
Catalog No. TMAB-00264
Anti-C5b-9 Polyclonal Antibody is a Rabbit antibody targeting C5b-9. Anti-C5b-9 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human,Rat (predicted:Mouse) |
| Ig Type | IgG |
| Reactivity | Human,Rat (predicted:Mouse) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (C5b-9) Polyclonal Antibody, Unconjugated (TMAB-00264) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 2. Sample: Lane 1: A549 (Human) Cell Lysate at 30 μg Lane 2: Hela (Human) Cell Lysate at 30 μg Primary: Anti-C5b (TMAB-00264) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 72 kDa Observed band size: 72 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Activation of the complement system plays a key role in normal inflammatory response to injury but may cause substantial injury when activated inappropriately. The complement system is activated either through the classical (antibody induced) or the alternative (microbial surface, polysacharride induced) pathway, both leading to the formation of the C5b9 complex. Fluid phase binding of the multifunctional glycoprotein S protein (vitronectin) to C5b9 leads to the formation of a cytolytically inactive complex, SC5b9, which is unable to attach to cells. |
| Immunogen | Purified Human SC5b-9 Complex |
| Antigen Species | Human |
| Gene ID | 735 |
| Uniprot ID | |
| Biology Area | Oxidative Stress,Alternative Pathway,Classical Pathway,MAC,Macrophage / Inflamm.,Oxidative stress,GPCR |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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