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AkaLumine hydrochloride

Name: AkaLumine hydrochloride
Brand: TargetMol
SKU: T18914
Price: 71 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T18914Cas No. 2558205-28-8

Alias AkaLumine hydrochloride (1176235-08-7 free base), AkaLumine hydrochloride

AkaLumine hydrochloride is a fluorescein analog with a Km value of 2.06 μM for recombinant fluorescent protein.

AkaLumine hydrochloride

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Purity: 99.32%

Catalog No. T18914Alias AkaLumine hydrochloride (1176235-08-7 free base), AkaLumine hydrochlorideCas No. 2558205-28-8

AkaLumine hydrochloride is a fluorescein analog with a Km value of 2.06 μM for recombinant fluorescent protein.

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$71	In Stock	In Stock
2 mg	$98	In Stock	In Stock
5 mg	$172	In Stock	In Stock
10 mg	$278	In Stock	In Stock
25 mg	$548	In Stock	In Stock
50 mg	$763	In Stock	In Stock
100 mg	$1,030	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.32%

Color:Red to Black

Resource Download

COA LCMS HNMR

Product Introduction

Bioactivity

Description	AkaLumine hydrochloride is a fluorescein analog with a Km value of 2.06 μM for recombinant fluorescent protein.
Targets&IC50	Recombinant Fluc protein:2.06 μM
In vitro	In both LLC/luc and MDA-MB-231/Luc cells exposed to AkaLumine hydrochloride, the signal peaks at lower concentrations (starting from 2.5 μM). Conversely, the bioluminescence produced by D-luciferin and CycLuc1 continuously increases with concentration, without reaching saturation, even at 250 μM.[1]
In vivo	AkaLumine hydrochloride-treated mice 15 min after intravenous injection of LLC/luc cells at the dose of 5 mM, exhibit a 3.3-fold increase in detection sensitivity of disseminated cancer cells in the lung compared with the experimental control group. In the inverse order at an 8 h interval. AkaLumine hydrochloride displays about a fourfold increase in signals from lung metastasis compared with the experimental control group.[1]
Animal Research	Instructions I. Solution preparation 1. Stock solution preparation Dissolve AkaLumine hydrochloride in deionized water or dimethyl sulfoxide (DMSO). 2. Working solution preparation: Prepare a stock solution of no more than 50 mg/mL, with a common concentration of 40 mg/mL. For example, 40 mg AkaLumine hydrochloride can be dissolved in 1 mL deionized water or DMSO. Notes: Storage: It is recommended to prepare and use immediately; if storage is required, it should be stored at low temperature to ensure stability. II. In vivo imaging: 1. Administration: Inject the prepared AkaLumine hydrochloride solution into experimental animals by intraperitoneal injection. 2. Dosage: The specific dose is adjusted according to the experimental situation, generally ranging from 50–200 mg/kg. 3. Imaging: After injection, use an imaging system that can detect NIR luminescence for bioluminescence imaging. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Synonyms	AkaLumine hydrochloride (1176235-08-7 free base), AkaLumine hydrochloride

Chemical Properties

Molecular Weight	338.85
Formula	C₁₆H₁₉ClN₂O₂S
Cas No.	2558205-28-8
Smiles	Cl.CN(C)c1ccc(\C=C\C=C\C2=N[C@H](CS2)C(O)=O)cc1
Relative Density.	no data available

Storage & Solubility Information

Storage

store at low temperature,keep away from direct sunlight,keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 120 mg/mL (354.14 mM), Sonication is recommended.

H2O: 25 mg/mL (73.78 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 5 mg/mL (14.76 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	2.9512 mL	14.7558 mL	29.5116 mL	147.5579 mL
5 mM	0.5902 mL	2.9512 mL	5.9023 mL	29.5116 mL
10 mM	0.2951 mL	1.4756 mL	2.9512 mL	14.7558 mL
20 mM	0.1476 mL	0.7378 mL	1.4756 mL	7.3779 mL
50 mM	0.0590 mL	0.2951 mL	0.5902 mL	2.9512 mL

DMSO

	1mg	5mg	10mg	50mg
100 mM	0.0295 mL	0.1476 mL	0.2951 mL	1.4756 mL

Calculator

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

recombinant Fluc proteinDye reagentAkaLumine Hydrochloride1176235-08-7 free base1176235-08-7