AkaLumine hydrochloride

AkaLumine hydrochloride is a luciferin analog, a luciferase substrate (Km=2.06 μM), emitting near-infrared light (λmax=677 nm) with high tissue penetration, used for deep tissue bioimaging research.

Recombinant Fluc protein:2.06 μM

Methods:Mouse lung cancer LLC/luc cells stably expressing firefly luciferase and human breast cancer MDA-MB-231/luc cells were used. AkaLumine hydrochloride was incubated at concentration gradients of 0.1–250 μM, and bioluminescence was detected by IVIS Spectrum 1 minute after substrate addition.
Results: This substrate reached maximum signal at a low concentration of 2.5 μM, whereas D-luciferin and CycLuc1 showed dose-dependent increases. [1]

Methods: Nude mice with bone metastasis models established by intracardiac injection of MDA-MB-231-luc cells were used. AkaLumine hydrochloride was administered by intravenous injection at a dose of 3 μmol as a single dose 0.25–2 hours before imaging; the solvent formulation was not specified.
Results: Fluorescence signals were detected in the chest and knee joints with AkaLumine hydrochloride, but the signals were relatively faint with lower resolution compared to P800SO3. [2]
Methods: The in vivo activity of AkaLumine hydrochloride was validated in a BALB/c nude mouse subcutaneous xenograft model of HEK 293T cells expressing different luciferases. AkaLumine hydrochloride was administered by intraperitoneal injection at a dose of 50 mg/kg (dissolved in 33 mM normal saline), with dynamic imaging performed within 30 minutes after substrate injection using 30-second acquisition intervals, with D-LH2 and others as substrate controls.
Results: The Akaluc/AkaLumine hydrochloride pair emitted light with a peak at 660 nm, while pairing with other luciferases reached the near-infrared region. The Akaluc/AkaLumine hydrochloride combination showed significantly higher luminescence brightness than Akaluc/D-LH2, and Luc2 paired with this substrate also exhibited relatively high luminescence signals. [3]

Instructions
I. Solution preparation
1. Stock solution preparation Dissolve AkaLumine hydrochloride in deionized water or dimethyl sulfoxide (DMSO).
2. Working solution preparation: Prepare a stock solution of no more than 50 mg/mL, with a common concentration of 40 mg/mL. For example, 40 mg AkaLumine hydrochloride can be dissolved in 1 mL deionized water or DMSO.
Notes: Storage: It is recommended to prepare and use immediately; if storage is required, it should be stored at low temperature to ensure stability.
II. In vivo imaging:
1. Administration: Inject the prepared AkaLumine hydrochloride solution into experimental animals by intraperitoneal injection.
2. Dosage: The specific dose is adjusted according to the experimental situation, generally ranging from 50–200 mg/kg.
3. Imaging: After injection, use an imaging system that can detect NIR luminescence for bioluminescence imaging.

The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.

DMSO: 120 mg/mL (354.14 mM), Sonication is recommended.

H2O: 25 mg/mL (73.78 mM), Sonication is recommended.

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 5 mg/mL (14.76 mM), Sonication is recommended.