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Ac-Gly-BoroPro

Name: Ac-Gly-BoroPro
Brand: TargetMol
SKU: T10227
Rating: 4.5 (1 reviews)

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Catalog No. T10227Cas No. 886992-99-0

Ac-Gly-BoroPro is a selective FAP inhibitor with a Ki of 23 nM.

Ac-Gly-BoroPro

😃Good

Catalog No. T10227Cas No. 886992-99-0

Ac-Gly-BoroPro is a selective FAP inhibitor with a Ki of 23 nM.

Pack Size	Price	USA Warehouse	Global Warehouse
25 mg	Inquiry	6-8 weeks	6-8 weeks
50 mg	Inquiry	6-8 weeks	6-8 weeks
100 mg	Inquiry	6-8 weeks	6-8 weeks

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Questions

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Product Introduction

Bioactivity

Description	Ac-Gly-BoroPro is a selective FAP inhibitor with a Ki of 23 nM.
Targets&IC50	FAP:ki:23 nM
In vitro	Ac-Gly-BoroPro selectively inhibits FAP relative to other prolyl peptidases. FAP reacts readily with submicromolar concentrations of Ac-Gly-BoroPro, reaching steady-state inhibition levels rapidly (Ki: 23 nM). In contrast, DPP-4 requires higher Ac-Gly-BoroPro concentrations for inhibition and a longer time to reach steady-state inhibition levels (Ki=377±18 nM). Ac-Gly-BoroPro inhibits other prolyl peptidases (DPP-7, DPP-8, DPP-9, prolyl oligopeptidase, and acylpeptide hydrolase) with Ki values ranging from 9- to 5400-fold higher than that for FAP inhibition.
Kinase Assay	Ki values for inhibition of proteases by Ac-Gly-BoroPro are determined using the method of progress curves for analysis of tight-binding competitive inhibitors. Various concentrations of Ac-Gly-BoroPro are reacted with FAP (1.0 nM) and DPP-4 (0.1 nM) in the presence of Ala-Pro-AFC (500 μM for FAP; 100 μM for DPP-4), and time-dependent inhibition of each protease is monitored. Reactions contained inhibitor concentrations at least 20-fold greater than protease concentrations, such that the protease-inhibitor complex does not significantly deplete the free inhibitor.

Chemical Properties

Molecular Weight	214.03
Formula	C₈H₁₅BN₂O₄
Cas No.	886992-99-0
Smiles	CC(=O)NCC(=O)N1CCC[C@@H]1B(O)O
Relative Density.	1.26 g/cm3 (Predicted)
Sequence	Ala-Cys-Gly-Arg-Pro
Sequence Short	G-{boroP}

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 50 mg/mL (233.61 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 2.5 mg/mL (11.68 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	4.6722 mL	23.3612 mL	46.7224 mL	233.6121 mL
5 mM	0.9344 mL	4.6722 mL	9.3445 mL	46.7224 mL
10 mM	0.4672 mL	2.3361 mL	4.6722 mL	23.3612 mL
20 mM	0.2336 mL	1.1681 mL	2.3361 mL	11.6806 mL
50 mM	0.0934 mL	0.4672 mL	0.9344 mL	4.6722 mL
100 mM	0.0467 mL	0.2336 mL	0.4672 mL	2.3361 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

FAPAc-Gly-BoroProAcGlyBoroProAc Gly BoroPro