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WAY 316606 HCl (915759-45-4 free base)

Catalog No. T23529 CAS T23529

Synonyms: WAY 316606 HCl

WAY 316606 HCl is a selective small-molecule inhibitor of secreted frizzled-related protein-1 (EC50: 0.65 μM).

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WAY 316606 HCl (915759-45-4 free base) Chemical Structure

WAY 316606 HCl (915759-45-4 free base), CAS T23529

Pack Size	Availability	Price/USD
5 mg	Inquiry	$ 181.00
10 mg	Inquiry	$ 288.00
50 mg	Inquiry	$ 1,146.00
100 mg	Inquiry	$ 1,832.00

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Biological Description

Chemical Properties

Storage & Solubility Information

Description	WAY 316606 HCl is a selective small-molecule inhibitor of secreted frizzled-related protein-1 (EC50: 0.65 μM).
In vitro	WAY 316606 HCl is a selective inhibitor of sFRP-1. It bound to sFRP-2 with 10-fold weaker affinity (Kd: 1 μM). In a fluorescence polarization binding assay, WAY 316606 HCl inhibited sFRP-1 (IC50: 0.5 μM). Although sequence of sFRP-1, -2 and -5 showed high identity, WAY 316606 HCl selectively inhibited sFRP-1 against other two proteins. In the osteosarcoma U2-OS cell line used in a cell-based transient transfection and luciferase reporter assay, treatment of WAY 316606 HCl dose-dependently increased Wnt-signaling (EC50: 0.65 μM). In an ex vivo assay using neonatal murine calvarium, treatment of WAY 316606 HCl dose-dependently enhanced the osteoblast activity and stimulated bone formation up to 60% (EC50: 1 nM) [1][2].
In vivo	Although WAY 316606 had good aqueous solubility and good stability, WAY 316606 HCl exerted high plasma clearance (77 mL/min/kg) in rats, which suppressed sustained exposure and limited the compound's advancement as an orally administered anabolic bone agent [1].

Synonyms	WAY 316606 HCl
Molecular Weight	484.94
Formula	C18H19F3N2O4S2·HCl
CAS No.	T23529

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: ≥24.25mg/mL

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

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