DCVJ (9-(2,2-Dicyanovinyl)julolidine) is a fluorescent dye showing high sensitivity to lag phase early amyloid β-peptide, and it is an identification of α-syn production.

Instructions
1. Preparation of stock solution and working solution:
1) Preparation of stock solution: DCVJ is usually dissolved in an organic solvent such as dimethyl sulfoxide (DMSO) to prepare a stock solution. The concentration of the stock solution is usually 1-10mM.
2) Preparation of working solution: The working solution is appropriately diluted according to the experimental purpose. If necessary, a concentration gradient can be set. The working concentration is usually between 1–10 µM.
2. Sample preparation and staining:
1) DCVJ is added to the sample (such as cells or tissue sections) for staining. The specific concentration depends on the experimental design.
2) The incubation time for staining will vary, usually 30 minutes to 1 hour, and the temperature is generally room temperature.
3. Detection:
1) DCVJ has a fluorescence absorption/emission spectrum at about 489/505 nm, which can be monitored by fluorescence microscopy or flow cytometry.
2) When monitoring cell degranulation (such as mast cells), changes in fluorescence intensity can reflect changes in molecular interactions or lipid bilayers.
4. Analysis: The rearrangement of microtubules and actin filaments, as well as changes in the lipid bilayer during cellular events such as degranulation, can be assessed by tracking changes in fluorescence intensity.
Notes:
1) DCVJ should be stored in a dark, cool place and protected from light as it is light sensitive and easily photobleached.
2) Appropriate protective gear such as gloves and lab coats should be worn when using it.

The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.

DMSO: 50 mg/mL (200.55 mM), Sonication is recommended.

Ethanol: < 1 mg/mL (insoluble)

DMF: 10 mg/mL (40.11 mM), Sonication is recommended.