ABMDMA (9,10-Anthracenediyl-bis(methylene)dimalonic acid) is a chemical probe and indicator with photobleaching properties that can be photobleached by mono-linear oxygen to the corresponding peroxylactone, and can be used to determine photo-induced mono-linear oxygen generation (SOG).

I. Singlet oxygen generation (SOG) measurement
1. Solution preparation: Dissolve ABMDMA in a suitable solvent (such as DMSO or aqueous buffer) at a working concentration of 1-10uM. Make sure the concentration of the probe is suitable for the specific experimental needs.
2. Singlet oxygen generation: Expose the sample to light (usually UV or visible light) to induce the generation of singlet oxygen.
3. Monitoring: After irradiation, measure the fluorescence or absorbance changes of ABMDMA to monitor singlet oxygen generation. The photobleaching changes of ABMDMA due to the reaction with singlet oxygen can directly reflect the generation of singlet oxygen.
II. Photochemical and photophysical studies
1. Excitation: Excite ABMDMA in the sample with an appropriate light source to excite the anthracene group.
2. Detection of photobleaching: Monitor the fluorescence quenching or absorbance changes caused by the reaction of singlet oxygen with ABMDMA.
3. Analysis: Calculate the photobleaching rate and the extent of singlet oxygen generation from the data.
III. Photosensitizer study
1. Introduce ABMDMA into the photosensitizer system and illuminate the system.
2. Measure the photobleaching rate of ABMDMA to quantitatively evaluate the generation of singlet oxygen.