4-Methylumbelliferyl oleate is a fluorescent substrate for lipases (Ex/Em=320/450 nm), which can be cleaved by lipase and can be used to measure pancreatic inhibitory activity.

Pancreatic lipase inhibitory activity was assessed by employing 4-Methylumbelliferyl (4-MU) oleate as the substrate. The assay mixture contained 0.1 mM 4-MU oleate (50 µL), McIlvaine buffer (20 µL of 0.1 M citrate-Na2HPO4, pH 7.4), and the test sample (5 µL). This was followed by the addition of porcine pancreatic lipase (25 µL), adjusting the total volume to 0.1 mL. Post incubation at 37℃ for 10 minutes, lipase-catalyzed liberation of 4-MU was quantified using fluorescence multi-detection reader at excitation and emission wavelengths of 320 nm and 450 nm, respectively [1].

4-Methylumbelliferyl (4-MU) oleate as a substrate to measure the inhibitory activity of compounds on pancreatic lipase
a. Reagent preparation: The reaction mixture includes 50 µL of 0.1 mM 4-MU oleate, 20 µL of McIlvane buffer (0.1 M citric acid-Na2HPO4, pH 7.4) and 5 µL of sample solution.
b. Operation steps:
1. Add 25 µL of porcine pancreatic lipase to the reaction mixture and adjust the total volume to 0.1 mL.
2. After the mixture is incubated at 37°C for 10 minutes, the amount of 4-MU released by lipase is measured by using a fluorescence multifunctional detector at an excitation wavelength of 320 nm and an emission wavelength of 450 nm.

The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.

DMSO: 80 mg/mL (181.56 mM), Sonication is recommended.

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 3.3 mg/mL (7.49 mM), Sonication is recommended.