The 45 kcal% High Fat Diet is a type of high-fat model feed designed to induce obesity and type 2 diabetes in animal models.

Before conducting formal experiments, it is recommended to use preliminary trials to determine optimal conditions, such as animal strain, age, dosage, frequency, duration, and time and indices for testing. A 45 kcal% High Fat Diet is effective for developing animal models for obesity and type 2 diabetes.

1. Obesity Induction Mechanism: High-fat diets increase the size and number of fat cells, thereby inducing obesity. Specific methods for modeling include: Rats: Sprague-Dawley • Male • 3 weeks old, feeding daily for 15 weeks. Mice: C57BL/6J • Male • 1 month old, feeding daily for 5 months. Note: 1) It is advised to adjust diets using a 24-hour fasting method or a gradual 7-day dietary change. 2) Replace feed every 2-3 days, discard old feed. 3) Use wood shavings bedding, changed 1-2 times a week. 4) Store refrigerated.

Indicators for Successful Modeling: Body weight in the high-fat (HF) diet group is 37% heavier compared to the normal diet group. Serum biochemistry shows elevated blood glucose, AST, and ALT. Fat metabolism indicators such as total cholesterol (T-CHO), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), and free fatty acids (FFA) are significantly increased. Histopathology reveals more frequent cytoplasmic microvacuolation in hepatocytes at the liver lobule center in high-fat diet rats. Mild inflammatory infiltration by neutrophils and monocytes, fibrosis, and severe hepatocyte ballooning degeneration are observed. Other indicators include consistently higher liver and fat pad weights in the high-fat diet group compared to the normal diet group. Related Products: 60 kcal% High Fat Diet. Countermeasure Products: /

2. Type 2 Diabetes Induction Mechanism: A combination of a high-fat diet and Streptozotocin injection is a common method for establishing rodent models of type 2 diabetes (T2DM) and has recently been used in research on diabetes-related cognitive disorders. Specific methods for modeling include: Rats: Sprague-Dawley • Male • 4 weeks old, with a feeding regimen of 4 weeks on a high-fat diet followed by a single injection of 30 mg/kg Streptozotocin. Note: 1) It is suggested to use a 24-hour fasting method or a gradual 7-day dietary adjustment. 2) After 4 weeks of high-fat diet feeding, administer intraperitoneal injection of a single dose of Streptozotocin (30 mg/kg) after overnight fasting. 3) Rats with tail vein blood glucose exceeding 16.7 mmol/L after 7 and 14 days post-injection are considered diabetic.

Indicators for Successful Modeling: Blood glucose levels exceed 300 mg/dL (16.7 mmol/L). There is a significant decline in weight following STZ administration.