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4-Nitrophenyl α-L-rhamnopyranoside is a substrate commonly employed in various biochemical assays to measure the activity of rhamnose-hydrolyzing enzymes such as α-L-rhamnosidase. The compound possesses unique chemical properties that enable it to be hydrolyzed by these enzymes, resulting in the formation of a yellow product known as p-nitrophenol. This characteristic makes it a valuable tool for detecting and quantifying rhamnose-hydrolyzing enzyme activity in biological samples or microbial cultures.

| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 mg | Inquiry | 7-10 days | 7-10 days | |
| 50 mg | Inquiry | 7-10 days | 7-10 days | |
| 100 mg | Inquiry | 7-10 days | 7-10 days |
| Description | 4-Nitrophenyl α-L-rhamnopyranoside is a substrate commonly employed in various biochemical assays to measure the activity of rhamnose-hydrolyzing enzymes such as α-L-rhamnosidase. The compound possesses unique chemical properties that enable it to be hydrolyzed by these enzymes, resulting in the formation of a yellow product known as p-nitrophenol. This characteristic makes it a valuable tool for detecting and quantifying rhamnose-hydrolyzing enzyme activity in biological samples or microbial cultures. |
| Molecular Weight | 285.25 |
| Formula | C12H15NO7 |
| Cas No. | 18918-31-5 |
| Smiles | O[C@H]([C@@H]([C@H]1O)O)[C@@H](O[C@H]1C)OC(C=C2)=CC=C2[N+]([O-])=O |
| Storage | keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
Dissolve 2 mg of the compound in 100 μL DMSO
to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO
stock solution to 400 μL PEG300
and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O
and mix thoroughly until a homogeneous solution is obtained.
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