Shopping Cart

Your shopping cart is currently empty

2-Thiouridine

Name: 2-Thiouridine
Brand: TargetMol
SKU: T35656
Price: 29 USD
Availability: InStock
Rating: 5 (10 reviews)

Copy Product Info

😃Good

Catalog No. T35656Cas No. 20235-78-3

2-Thiouridine (s2U) is a naturally occurring modified nucleobase present in transfer RNAs that stabilizes U:A base pairing while modestly destabilizing U:G wobble interactions. 2-Thiouridine influences codon–anticodon recognition, translational accuracy, and RNA conformational dynamics, and is extensively used in studies of nucleic acid structure, RNA biology, and the engineering of functional RNA-based biomolecules.

2-Thiouridine

Copy Product Info

😃Good

Catalog No. T35656Cas No. 20235-78-3

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
25 mg	$29	-	In Stock
1 mL x 10 mM (in DMSO)	$29	-	In Stock

Add to Cart

Add to Quotation

In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Questions

Batch Information

Select Batch

Appearance:Solid

Color:White

Resource Download

COA HNMR

Product Introduction

Bioactivity

Description	2-Thiouridine (s2U) is a naturally occurring modified nucleobase present in transfer RNAs that stabilizes U:A base pairing while modestly destabilizing U:G wobble interactions. 2-Thiouridine influences codon–anticodon recognition, translational accuracy, and RNA conformational dynamics, and is extensively used in studies of nucleic acid structure, RNA biology, and the engineering of functional RNA-based biomolecules.
In vitro	In viral replication assays, 2-Thiouridine inhibited the replication of several positive-strand RNA viruses (DENV2, ZIKV, SARS-CoV, SARS-CoV-2) with effective concentrations ranging from 0.625 to 40 μM [1].
In vivo	In DENV2-infected mice, oral administration of 2-thiouridine (50-150 mg/kg, twice daily) prolonged survival and reduced viral RNA loads in the serum and spleen. In SARS-CoV-2 mouse models, treatment via intravenous injection (2-20 mg/kg, once daily) or high-dose oral administration (300 mg/kg, twice daily) for 5 days resulted in increased survival rates, lower lung viral titers, and reduced levels of inflammatory cytokines [4].

Chemical Properties

Molecular Weight	260.27
Formula	C₉H₁₂N₂O₅S
Cas No.	20235-78-3
Smiles	O[C@H]1[C@@H](O[C@H](CO)[C@H]1O)N2C(=S)NC(=O)C=C2

Storage & Solubility Information

Storage

store at low temperature | In solvent: -80°C for 1 year

Solubility Information

H2O: 8 mg/mL (30.74 mM), Sonication is recommended.

Ethanol: 2 mg/mL (7.68 mM), Sonication is recommended.

PBS (pH 7.2): 5 mg/mL (19.21 mM), Sonication is recommended.

DMF: 10 mg/mL (38.42 mM), Sonication is recommended.

DMSO: 40 mg/mL (153.69 mM), Sonication is recommended.

Solution Preparation Table

PBS (pH 7.2)/H2O/DMF/DMSO

	1mg	5mg	10mg	50mg
10 mM	0.3842 mL	1.9211 mL	3.8422 mL	19.2108 mL

H2O/DMF/DMSO

	1mg	5mg	10mg	50mg
20 mM	0.1921 mL	0.9605 mL	1.9211 mL	9.6054 mL

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

2-Thiouridine2Thiouridine2 Thiouridine