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VEGF165 Protein, Danio rerio (zebrafish), Recombinant
(Synonyms: vascular endothelial growth factor A) Copy Product InfoSynonyms: vascular endothelial growth factor A
Catalog No. TMPY-03080 Copy Product Info
VEGF165 Protein, Danio rerio (zebrafish), Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 19.2 kDa and the accession number is O73682.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $95 | 7-10 days | 7-10 days | |
| 10 µg | $153 | 7-10 days | 7-10 days | |
| 20 µg | $257 | 7-10 days | 7-10 days | |
| 50 µg | $496 | 7-10 days | 7-10 days | |
| 100 µg | $986 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 30-250ng/mL. |
| Description | VEGF165 Protein, Danio rerio (zebrafish), Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 19.2 kDa and the accession number is O73682. |
| Species | Danio rerio (zebrafish) |
| Expression System | Baculovirus Insect Cells |
| Tag | Tag Free |
| Accession Number | O73682 |
| Construction | A DNA sequence encoding the zebrafish VEGF165 (O73682-Isoform VEGF165) (Met 1-Arg188) was expressed and purified. Predicted N terminal: Ala 24 |
| Protein Purity | > 90 % as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, pH 7.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| Synonyms | vascular endothelial growth factor A |
| Research Background | Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and often exists as a disulfide-linked homodimer. VEGF-A protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, inhibiting apoptosis and tumor growth. VEGF-A protein is also a vasodilator that increases microvascular permeability, thus it was originally referred to as vascular permeability factor.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
| Molecular Weight | 19.2 kDa (predicted); 22 kDa (reducing conditions) |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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Related Tags: VEGF165 Protein, Danio rerio (zebrafish), Recombinant chemical structure | VEGF165 Protein, Danio rerio (zebrafish), Recombinant molecular weight
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