UCHL1 Protein, Mouse, Recombinant (His)

Name: UCHL1 Protein, Mouse, Recombinant (His)
Brand: TargetMol
SKU: TMPY-02175
Price: 58 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: UCHL-1, UCH-L1, ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase), R75593, PGP9.5, gad, C88048, AW822034) Copy Product Info

Synonyms: UCHL-1, UCH-L1, ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase), R75593, PGP9.5, gad, C88048, AW822034

Catalog No. TMPY-02175 Copy Product Info

UCHL1 Protein, Mouse, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 26.2 kDa and the accession number is Q9R0P9.

Pack Size	Price	USA Stock	Global Stock
5 µg	$58	-	In Stock
10 µg	$93	-	In Stock
20 µg	$152	-	In Stock
50 µg	$298	7-10 days	7-10 days
100 µg	$577	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:≥ 95 % as determined by SDS-PAGE

Appearance:Liquid

Color:Transparent

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Measured by the hydrolysis of UbiquitinAMC. The specific activity is >100 pmoles/min/μg.
Description	UCHL1 Protein, Mouse, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 26.2 kDa and the accession number is Q9R0P9.
Species	Mouse
Expression System	E. coli
Tag	N-His
Accession Number	Q9R0P9
Construction	A DNA sequence encoding the mouse UCHL1 (Q9R0P9) (Gln 2-Ala 223) was expressed, with a polyhistide tag at the N-terminus. Predicted N terminal: Met
Protein Purity	> 97 % as determined by SDS-PAGE
Endotoxin	Please contact us for more information.
Formulation	Supplied as sterile 50 mM Tris, 150 mM NaCl, 20% glycerol, pH 7.7.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	UCHL-1, UCH-L1, ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase), R75593, PGP9.5, gad, C88048, AW822034
Research Background	Ubiquitin carboxyl-terminal hydrolase isozyme L1, also known as UCH-L1, Ubiquitin thioesterase L1, PGP9.5 and UCHL1, is a deubiqutinating enzyme with important functions in recycling of ubiquitin. Regulated proteolysis by the ubiquitin pathway has been implicated in control of the cell cycle, transcriptional activation, cell fate and growth, and synaptogenesis. The ubiquitin-proteasome system is involved in synaptic plasticity and is proposed to be part of a molecular switch that converts short-term synaptic potentiation to long-term changes in synaptic strength. UCHL1 is found in neuronal cell bodies and processes throughout the neocortex (at protein level). It is expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. UCHL1 is weakly expressed in ovary. UCHL1 is a ubiquitin-protein hydrolase. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. UCHL1 also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity. UCHL1 dysfunction has been associated with neurodegeneration in Parkinson's, Alzheimer's, and Huntington's disease patients. Reduced UCHL1 function may jeopardize the survival of CNS neurons.

Chemical Properties

Molecular Weight

26.2 kDa (predicted); 29 kDa (reducing conditions)

Storage & Solubility Information

Shipping	Proteins are shipped with blue ice.
Storage	It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.