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UCHL3 Protein, Rat, Recombinant (His)
(Synonyms: ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)) Copy Product InfoSynonyms: ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
Catalog No. TMPY-02332 Copy Product Info
UCHL3 Protein, Rat, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 27.5 kDa and the accession number is Q91Y78.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $108 | 7-10 days | 7-10 days | |
| 10 μg | $178 | 7-10 days | 7-10 days | |
| 20 μg | $296 | 7-10 days | 7-10 days | |
| 50 μg | $696 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Measured by the hydrolysis of UbiquitinAMC. The specific activity is >14000 pmoles/min/μg. |
| Description | UCHL3 Protein, Rat, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 27.5 kDa and the accession number is Q91Y78. |
| Species | Rat |
| Expression System | E. coli |
| Tag | N-His |
| Accession Number | Q91Y78 |
| Construction | A DNA sequence encoding the rat UCHL3 (Q91Y78) (Glu 2-Ala 230) was fused with a polyhistidine tag at the N-terminus. Predicted N terminal: Met |
| Protein Purity | > 95 % as determined by SDS-PAGE |
| Endotoxin | Please contact us for more information. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) |
| Research Background | Ubiquitin carboxyl-terminal hydrolase isozyme L3, also known as UCH-L3, Ubiquitin thioesterase L3 and UCHL3, is a ubiquitin-protein hydrolase that belongs to the peptidase C12 family. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. UCHL3 is highly expressed in heart, skeletal muscle, and testis. UCHL1 and UCHL3 are two of the deubiquitinating enzymes expressed in the brain. These phenotypes indicate the importance of UCHL1 and UCHL3 in the regulation of the central nervous system. UCHL3 functions as a de-ubiquitinating enzyme where lack of its hydrolase activity may result in the prominent accumulation of ubiquitinated proteins and subsequent induction of stress responses in skeletal muscle. UCHL3 has also been identified as a tumor-specific antigen in colon cancer. |
| Molecular Weight | 27.5 kDa (predicted); 28 kDa (reducing conditions) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: UCHL3 Protein, Rat, Recombinant (His) chemical structure | UCHL3 Protein, Rat, Recombinant (His) molecular weight
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