SKP1 Protein, Human, Recombinant (His, Avi)

The S-phase Kinase-Associated Protein 1 (SKP1) is a core component of the SKP1, Cullin 1, F-box protein (SCF) complex, an E3 ubiquitin ligase that serves to poly-ubiquitinate a vast array of protein targets as a signal for their proteasomal degradation, thereby playing a critical role in the regulation of downstream biological processes. SKP1 Protein, Human, Recombinant (His, Avi) is expressed in HEK293 mammalian cells with N-His and C-Avi tag. The predicted molecular weight is 21.43 kDa and the accession number is P63208-1.

Pack Size	Availability	Price/USD
100 μg	5 days	$ 418.00
500 μg	5 days	$ 1,670.00
1 mg	5 days	$ 2,800.00

Pack Size

Availability

Price/USD

Quantity

100 μg

5 days

$ 418.00

500 μg

5 days

$ 1,670.00

1 mg

5 days

$ 2,800.00

Description

Species	Human
Expression System	HEK293 Cells
Tag	N-His, C-Avi
Accession Number	P63208-1
Synonyms	OCP-II, TCEB1L, SIII, p19A, OCP-2, EMC19, OCP2, SKP1A
Construction	Pro2-Lys163
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	21.43 kDa (predicted). Due to glycosylation, the protein migrates to 23-28 kDa based on Tris-Bis PAGE result.

Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	The S-phase Kinase-Associated Protein 1 (SKP1) is a core component of the SKP1, Cullin 1, F-box protein (SCF) complex, an E3 ubiquitin ligase that serves to poly-ubiquitinate a vast array of protein targets as a signal for their proteasomal degradation, thereby playing a critical role in the regulation of downstream biological processes.