Siglec-8 Protein, Human, Recombinant (His)

Name: Siglec-8 Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPJ-01360
Rating: 4.5 (1 reviews)

(Synonyms: Siglec-8, Siglec8, SAF2SAF-2, SAF-2, SAF2, CDw329, CD329 antigen) Copy Product Info

Synonyms: Siglec-8, Siglec8, SAF2SAF-2, SAF-2, SAF2, CDw329, CD329 antigen

Catalog No. TMPJ-01360 Copy Product Info

Siglec-8 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 40-60 KDa and the accession number is Q9NYZ4.

Pack Size	Price	USA Stock	Global Stock
5 µg	$55	7-10 days	7-10 days
10 µg	$86	7-10 days	7-10 days
20 µg	$137	7-10 days	7-10 days
50 µg	$262	7-10 days	7-10 days
100 µg	$488	7-10 days	7-10 days
200 µg	$923	7-10 days	7-10 days
500 µg	$2,150	7-10 days	7-10 days
1 mg	$3,220	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	Siglec-8 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 40-60 KDa and the accession number is Q9NYZ4.
Species	Human
Expression System	HEK293 Cells
Tag	C-6xHis
Accession Number	Q9NYZ4
Amino Acid	Met17-Ala363
Construction	Met17-Ala363
Protein Purity	Greater than 95% as determined by reducing SDS-PAGE. Greater than 95% as determined by SEC-HPLC.
Endotoxin	< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	Siglec-8, Siglec8, SAF2SAF-2, SAF-2, SAF2, CDw329, CD329 antigen
Research Background	Siglec-8 is also known as SIGLEC8, SAF2, SIGLEC-8, SIGLEC8L and sialic acid binding Ig like lectin 8, is an approximately 75 kDa transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules. Siglec-8 is expressed on eosinophils, basophils, and mast cells, and it shows a binding preference for the carbohydrate 6-O sulfated sLex. At the tissue level, Siglec-8 mRNA was found to be most highly expressed in lung, PBMCs, spleen, and kidney. Mature human Siglec-8 consists of a 347 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 115 aa cytoplasmic domain with two tyrosine based signaling motifs. Alternative splicing generates additional isoforms that either lack most of the second Ig-like domain or have a substituted cytoplasmic domain without the signaling motifs. Cross-linking of Siglec-8 inhibits Fc epsilon RI alpha induced mast cell degranulation (9). It also induces eosinophil apoptosis, an effect which is enhanced by the eosinophil-activating cytokines IL-5, IL-33, and GM-CSF.

Chemical Properties

Molecular Weight

40-60 KDa (reducing condition)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.