Siglec-8 Protein, Human, Recombinant (hFc)

Siglec-8 is also known as SIGLEC8, SAF2, SIGLEC-8, SIGLEC8L and sialic acid binding Ig like lectin 8, is an approximately 75 kDa transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules. Siglec-8 is expressed on eosinophils, basophils, and mast cells, and it shows a binding preference for the carbohydrate 6-O sulfated sLex. At the tissue level, Siglec-8 mRNA was found to be most highly expressed in lung, PBMCs, spleen, and kidney. Mature human Siglec-8 consists of a 347 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 115 aa cytoplasmic domain with two tyrosine based signaling motifs. Alternative splicing generates additional isoforms that either lack most of the second Ig-like domain or have a substituted cytoplasmic domain without the signaling motifs. Cross-linking of Siglec-8 inhibits Fc epsilon RI alpha induced mast cell degranulation (9). It also induces eosinophil apoptosis, an effect which is enhanced by the eosinophil-activating cytokines IL-5, IL-33, and GM-CSF.

Pack Size	Availability	Price/USD
10 μg	5 days	$ 86.00
50 μg	5 days	$ 262.00
500 μg	5 days	$ 2,150.00
1 mg	5 days	$ 3,220.00

Pack Size

Availability

Price/USD

Quantity

10 μg

5 days

$ 86.00

50 μg

5 days

$ 262.00

500 μg

5 days

$ 2,150.00

1 mg

5 days

$ 3,220.00

Description

Species	Human
Expression System	Human Cells
Tag	C-Fc
Accession Number	Q9NYZ4
Synonyms	SAF2, CD329 antigen, CDw329, Siglec-8, SAF2SAF-2, SAF-2, Siglec8
Amino Acid	Met17-Ala363
Construction	Recombinant Human Sialic Acid-binding Ig-like Lectin 8 is produced by our Mammalian expression system and the target gene encoding Met17-Ala363 is expressed with a human IgG1 Fc tag at the C-terminus.
Protein Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Molecular Weight	80-100 KDa, reducing conditions

Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Stability & Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Research Background	Siglec-8 is also known as SIGLEC8, SAF2, SIGLEC-8, SIGLEC8L and sialic acid binding Ig like lectin 8, is an approximately 75 kDa transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules. Siglec-8 is expressed on eosinophils, basophils, and mast cells, and it shows a binding preference for the carbohydrate 6-O sulfated sLex. At the tissue level, Siglec-8 mRNA was found to be most highly expressed in lung, PBMCs, spleen, and kidney. Mature human Siglec-8 consists of a 347 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 115 aa cytoplasmic domain with two tyrosine based signaling motifs. Alternative splicing generates additional isoforms that either lack most of the second Ig-like domain or have a substituted cytoplasmic domain without the signaling motifs. Cross-linking of Siglec-8 inhibits Fc epsilon RI alpha induced mast cell degranulation (9). It also induces eosinophil apoptosis, an effect which is enhanced by the eosinophil-activating cytokines IL-5, IL-33, and GM-CSF.

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Siglec-8 Protein, Human, Recombinant (hFc)

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