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NAMPT Protein, Human, Recombinant (His)

(Synonyms: Visfatin, Pre-B-cell colony-enhancing factor 1, Pre-B cell-enhancing factor, PBEF1, PBEF, Nicotinamide phosphoribosyltransferase, NAMPT, NAmPRTase) Copy Product Info

Synonyms: Visfatin, Pre-B-cell colony-enhancing factor 1, Pre-B cell-enhancing factor, PBEF1, PBEF, Nicotinamide phosphoribosyltransferase, NAMPT, NAmPRTase

Catalog No. TMPJ-00968 Copy Product Info
Pre-B cell colony enhancing factor (PBEF) was originally identified as a cytokine that potentiated the clonal expansion and differentiation of pre-B cells, but it is also acknowledged to be the ubiquitous intracellular enzyme nicotinamide phosphoribosyltranferase (NAMPT) and the adipokine "visfatin". PBEF is constitutively expressed in the fetal membranes where its greatest expression is in the amnion. It has intracellular and extracellular forms. Most of the intracellular functions of PBEF are due to its role as a Nampt which can induce angiogenesis through upregulation of VEGF and VEGFR and secretion of MCP-1. Extracellular PBEF has been shown to increase inflammatory cytokines, such as TNF-α, IL-1β, IL-16, and TGF-β1. PBEF also increases the production of IL-6, TNF-α, and IL-1β in CD14+ monocyctes, macrophages, and dendritic cells, enhances the effectiveness of T cells.
Pack SizePriceUSA StockGlobal StockQuantity
5 μg$727-10 days7-10 days
10 μg$1167-10 days7-10 days
20 μg$1767-10 days7-10 days
50 μg$3107-10 days7-10 days
100 μg$5327-10 days7-10 days
200 μg$9197-10 days7-10 days
500 μg$1,9007-10 days7-10 days
1 mg$2,7307-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Batch Information

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Purity:94%
Appearance:Lyophilized powder
Color:White
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Product Introduction

Bioactivity
Bioactivity
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
Pre-B cell colony enhancing factor (PBEF) was originally identified as a cytokine that potentiated the clonal expansion and differentiation of pre-B cells, but it is also acknowledged to be the ubiquitous intracellular enzyme nicotinamide phosphoribosyltranferase (NAMPT) and the adipokine "visfatin". PBEF is constitutively expressed in the fetal membranes where its greatest expression is in the amnion. It has intracellular and extracellular forms. Most of the intracellular functions of PBEF are due to its role as a Nampt which can induce angiogenesis through upregulation of VEGF and VEGFR and secretion of MCP-1. Extracellular PBEF has been shown to increase inflammatory cytokines, such as TNF-α, IL-1β, IL-16, and TGF-β1. PBEF also increases the production of IL-6, TNF-α, and IL-1β in CD14+ monocyctes, macrophages, and dendritic cells, enhances the effectiveness of T cells.
Species
Human
Expression System
E. coli
TagN-6xHis
Accession NumberP43490
Amino AcidMet1-His491
ConstructionMet1-His491
Protein Purity
Greater than 90% as determined by reducing SDS-PAGE. (QC verified)
NAMPT Protein, Human, Recombinant (His)
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM PB, 10% Trehalose, 100 mM Arg-HCl, 0.02% Tween 80, pH6.0.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & StorageLyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
SynonymsVisfatin, Pre-B-cell colony-enhancing factor 1, Pre-B cell-enhancing factor, PBEF1, PBEF, Nicotinamide phosphoribosyltransferase, NAMPT, NAmPRTase
Research Background
Chemical Properties
Molecular Weight55 KDa (reducing condition)
Storage & Solubility Information
StorageStore at low temperature Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Keywords

Related Tags: NAMPT Protein, Human, Recombinant (His) chemical structure | NAMPT Protein, Human, Recombinant (His) molecular weight