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LILRB1/CD85j/ILT2 Protein, Rhesus macaque, Recombinant (aa 17-456, hFc)

(Synonyms: XXbac-BCX85G21.4, MIR7, LIR1, LILRB1, ILT2FLJ37515, ILT-2, ILT2, CD85J) Copy Product Info

Synonyms: XXbac-BCX85G21.4, MIR7, LIR1, LILRB1, ILT2FLJ37515, ILT-2, ILT2, CD85J

Catalog No. TMPK-00648 Copy Product Info
LILRB1/CD85j/ILT2 Protein, Rhesus macaque, Recombinant (aa 17-456, hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 74.5 kDa and the accession number is F7H3G7.

Product Introduction

Bioactivity
Bioactivity
Anti-LILRB1 Antibody immobilized on CM5 Chip can bind Rhesus macaque LILRB1, hFc Tag with an affinity constant of 51.44 nM as determined in SPR assay.
Description
LILRB1/CD85j/ILT2 Protein, Rhesus macaque, Recombinant (aa 17-456, hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 74.5 kDa and the accession number is F7H3G7.
Species
Rhesus
Expression System
HEK293 Cells
TagC-hFc
Accession NumberF7H3G7
ConstructionSer17-His456
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
SynonymsXXbac-BCX85G21.4, MIR7, LIR1, LILRB1, ILT2FLJ37515, ILT-2, ILT2, CD85J
Research Background
LILRB1, also known as CD85j and ILT2, is a 110 kDa transmembrane glycoprotein in the LILR immunoregulatory protein family. Mature human LILRB1 consists of a 438 amino acid (aa) extracellular domain (ECD) with 4 tandem Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 4 inhibitory ITIM motifs.LILRB1 is a receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles. Receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response.
Chemical Properties
Molecular Weight74.5 kDa (predicted). Due to glycosylation, the protein migrates to 80-90 kDa based on Tris-Bis PAGE result.
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

Related Tags: LILRB1/CD85j/ILT2 Protein, Rhesus macaque, Recombinant (aa 17-456, hFc) chemical structure | LILRB1/CD85j/ILT2 Protein, Rhesus macaque, Recombinant (aa 17-456, hFc) molecular weight