KRAS Protein, Human, Recombinant (G12V, His)

Name: KRAS Protein, Human, Recombinant (G12V, His)
Brand: TargetMol
SKU: TMPJ-00511
Rating: 4.5 (1 reviews)

(Synonyms: RASK2, KRAS2, Ki-Ras, c-K-ras, CFC2) Copy Product Info

Synonyms: RASK2, KRAS2, Ki-Ras, c-K-ras, CFC2

Catalog No. TMPJ-00511 Copy Product Info

KRAS Protein, Human, Recombinant (G12V, His) is expressed in E. coli expression system with N-6xHis tag. The predicted molecular weight is 25-30 KDa and the accession number is AAH13572.1.

Pack Size	Price	USA Stock	Global Stock
5 µg	$34	7-10 days	7-10 days
10 µg	$50	7-10 days	7-10 days
20 µg	$80	7-10 days	7-10 days
50 µg	$156	7-10 days	7-10 days
100 µg	$252	7-10 days	7-10 days
200 µg	$397	7-10 days	7-10 days
500 µg	$790	7-10 days	7-10 days
1 mg	$1,190	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:Greater than 95% as determined by reducing SDS-PAGE.

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	KRAS Protein, Human, Recombinant (G12V, His) is expressed in E. coli expression system with N-6xHis tag. The predicted molecular weight is 25-30 KDa and the accession number is AAH13572.1.
Species	Human
Expression System	E. coli
Tag	N-6xHis
Accession Number	AAH13572.1
Amino Acid	Thr2-Cys185(Gly12Val)
Construction	Thr2-Cys185(Gly12Val)
Protein Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Endotoxin	< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	RASK2, KRAS2, Ki-Ras, c-K-ras, CFC2
Research Background	K-Ras belongs to the small GTPase superfamily, Ras family. As other members of the Ras family, K-Ras is a GTPase and is an early player in many signal transduction pathways. It is usually tethered to cell membranes because of the presence of an isoprenyl group on its C-terminus. K-Ras functions as a molecular on/off switch. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation. Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner. Besides essential function in normal tissue signaling, the mutation of a K-Ras gene is an essential step in the development of many cancers. Several germline K-Ras mutations have been found to be associated with Noonan syndrome[4] and cardio-facio-cutaneous syndrome. Somatic K-Ras mutations are found at high rates in Leukemias, colon cancer, pancreatic cancer and lung cancer.

Chemical Properties

Molecular Weight

25-30 KDa (reducing condition)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.