KRAS Protein, Human, Recombinant (G12D, His)

Name: KRAS Protein, Human, Recombinant (G12D, His)
Brand: TargetMol
SKU: TMPY-06056
Price: 52 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: RASK2, RALD, NS3, NS, K-RAS4B, K-RAS4A, K-RAS2B, K-RAS2A, KRAS2, KRAS1, K-RAS, Kirsten rat sarcoma viral oncogene homolog, KI-RAS, C-K-RAS, CFC2) Copy Product Info

Synonyms: RASK2, RALD, NS3, NS, K-RAS4B, K-RAS4A, K-RAS2B, K-RAS2A, KRAS2, KRAS1, K-RAS, Kirsten rat sarcoma viral oncogene homolog, KI-RAS, C-K-RAS, CFC2

Catalog No. TMPY-06056 Copy Product Info

KRAS Protein, Human, Recombinant (G12D, His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 22 kDa and the accession number is P01116-2.

Pack Size	Price	USA Stock	Global Stock
5 µg	$52	-	In Stock
10 µg	$81	-	In Stock
20 µg	$129	-	In Stock
50 µg	$255	-	In Stock
100 µg	$450	7-10 days	7-10 days
200 µg	$793	7-10 days	7-10 days
500 µg	$1,670	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:92.5%

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	The specific activity of KRAS was measured by GTPase-Glo assay.
Description	KRAS Protein, Human, Recombinant (G12D, His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 22 kDa and the accession number is P01116-2.
Species	Human
Expression System	E. coli
Tag	N-His
Accession Number	P01116-2
Construction	A DNA sequence encoding the human KRAS (P01116-2) (Thr2-Cys185, with natural variant Gly 12 Asp) was expressed with a polyhistidine tag at the N-terminus. Predicted N terminal: Met
Protein Purity	> 95 % as determined by SDS-PAGE.
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 25 mM Tris, 100 mM glycine, 10% glycerol, pH 7.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.15 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	RASK2, RALD, NS3, NS, K-RAS4B, K-RAS4A, K-RAS2B, K-RAS2A, KRAS2, KRAS1, K-RAS, Kirsten rat sarcoma viral oncogene homolog, KI-RAS, C-K-RAS, CFC2
Research Background	K-Ras belongs to the small GTPase superfamily, Ras family. Like other members of the Ras family, K-Ras is a GTPase and is an early player in many signal transduction pathways. It is usually tethered to cell membranes because of the presence of an isoprenyl group on its C-terminus. K-Ras functions as a molecular on/off switch. Once it is turned on it recruits and activates proteins necessary for the propagation of growth factor and other receptors' signal, such as c-Raf and PI 3-kinase. It binds to GTP in the active state and possesses an intrinsic enzymatic activity that cleaves the terminal phosphate of the nucleotide converting it to GDP. Upon conversion of GTP to GDP, K-Ras is turned off. The rate of conversion is usually slow but can be sped up dramatically by an accessory protein of the GTPase activating protein class, for example, RasGAP. In turn, K-Ras can bind to proteins of the Guanine Nucleotide Exchange Factor class, for example, SOS1, which forces the release of bound nucleotide. Subsequently, K-Ras binds GTP present in the cytosol and the GEF is released from ras-GTP. Besides essential function in normal tissue signaling, the mutation of a K-Ras gene is an essential step in the development of many cancers. Several germline K-Ras mutations are associated with Noonan syndrome and Cardio-Facio-Cutaneous syndrome. Somatic K-Ras mutations are found at high rates in Leukemias, colon cancer, pancreatic cancer, and lung cancer.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

Chemical Properties

Molecular Weight

22 kDa (predicted)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.